FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_75.35100000044ca4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_75.35100000044ca4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC96.870229007633588No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT53.816793893129771No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGTACAATCTCGTATGCCGTC21.5267175572519083No Hit
GAGGGTGGTGAAGATGGAGGAGCTGCTCAGGCCGAGGGAGGTTCATGCAAC10.7633587786259541No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGC10.7633587786259541No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATCATAAACACAT10.7633587786259541RNA PCR Primer, Index 36 (96% over 29bp)
ACCCGTCTGATAGCGCTTAAGCGCGAACTTCGAAAGCTGTCTCTTATACAC10.7633587786259541No Hit
GTTGTACCCATATGGTTCTTAGCCATTATCTTCCCCTTTTTCGGACCAATC10.7633587786259541No Hit
GACAAATATGGAAACAAATACTATGAAGACAACAAGCAATTTTTTGGTGCT10.7633587786259541No Hit
TGAACAGGGGTCACTTAACTTATCTCCTTTACTCCATTTTCTGTCTCTTAT10.7633587786259541No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGCACTATCTCGTATGCCGTC10.7633587786259541No Hit
CCCACGTCCCTAGGTCGACCATAGGACCTATCGCCGCCGCATGCAACACGG10.7633587786259541No Hit
AAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGACTGTCTCTTATA10.7633587786259541No Hit
GTACGGGAGGCTGAGGCGTGATGACGAGGCACTACGGTGCTGAAGCAACAA10.7633587786259541No Hit
ATCCAAGGATTCCCTTGAGTTTGCCTTCGGATTCCTCCTTGATCTGTCTCT10.7633587786259541No Hit
ATTCCAGACACATAAGAATCCAGCTAATGCCCAAATACACAGAGGAATAAT10.7633587786259541No Hit
TAACAGATCTGTCCTAGTGATTTTACCTTTGTTCTAGAACTGTCTCTTATA10.7633587786259541No Hit
ATGGTGATCTAGGTGTCCTAATTAGTTATCGTAAAGCTGTCTCTTATACAC10.7633587786259541No Hit
CTTGAGAGATGGAGAAAGAATTGATAGATGATTTGATGTGTTTGAACTGTC10.7633587786259541No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATCGGTTAATGA10.7633587786259541No Hit
GTACTTTTTTTTTTTTTTTTTTTTGTGTTTTTTCCACTTTTTTCTTATAAA10.7633587786259541No Hit
ACTGAGTACTTTGTGAATTGTGAATTGTTTCAGAATTGTGAAAATCAAGCC10.7633587786259541No Hit
GGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7633587786259541No Hit
CTGTACCCATATGTTACTGGAACTTCAATTGTTGCAATCAAGTACAAAGAT10.7633587786259541No Hit
TGCGTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGAACAAGAGA10.7633587786259541RNA PCR Primer, Index 31 (96% over 28bp)
GACATGGATTCTCCTAGGCCGTTTGGGTGGCGGTAGAGTCGTCCTGCGCAC10.7633587786259541No Hit
GTACATCAGCTGTGTAGAAAGAACAATTAATCTGATGGTTATTTTCTCTGG10.7633587786259541No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTGACCATCTCGTATGCCGTC10.7633587786259541No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGCCCCC10.7633587786259541No Hit
CTGCATAATCTACCAATCTCTTCAAGTAATTGACCTCTCCTCACATATCTC10.7633587786259541No Hit
CCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCTG10.7633587786259541No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCGGACCATCTCGTATGCCGTC10.7633587786259541No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT10.7633587786259541No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGCGGATTACG10.7633587786259541RNA PCR Primer, Index 27 (96% over 28bp)
TTCCAGTCTCAGAACCAGAACCGGAACCAGAATCAGAACCTGTCTCTTATA10.7633587786259541No Hit
CTCTTATCACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT10.7633587786259541No Hit
GGATTCTACTAGTCTGTTGCTTCCCTTTTGTTTTGATGAATTCTCAACTTT10.7633587786259541No Hit
ATCAACGCAGAGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC10.7633587786259541No Hit
GCTAAGAAGTCAGCTAAGAAATCTAGGGTTAATTGTTTCATCAAGCTTGTT10.7633587786259541No Hit
GTACATGCTTGTCCTCACACACTAATGCCGATGAGGAAAGCGTTTAAGGGG10.7633587786259541No Hit
GTTCCAGTAGTGACTGATTCACATTTTTTTCCAAATGTAATGCACACTCCA10.7633587786259541No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAATA10.7633587786259541RNA PCR Primer, Index 27 (96% over 28bp)
GCATAAAATGCCTTTTGTCTCACTTTGTTCCAGAGTAGATAGATACCGCAA10.7633587786259541No Hit
GTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCA10.7633587786259541No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTTCACGGGGA10.7633587786259541RNA PCR Primer, Index 27 (96% over 28bp)
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCCTGTCTCTTA10.7633587786259541No Hit
TCTATCCATGGTGTTTCGGGTAGCGGTTTCACACAGACGTTTGAGATTGAT10.7633587786259541No Hit
AACTTAGAGAGTATTACAACAACTTCCAAACATGGAAGGAAAAACACAGGA10.7633587786259541No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAA10.7633587786259541No Hit
GATGAATGTTGTTAAAGATTCCGTAATCCCTATCAATTGTTGTTAATCCTC10.7633587786259541No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAACGGGCCC10.7633587786259541No Hit
TAACTGTTTTTTCTTCTCACTTGTTTCTCTTAAGACCCTGTCTCTTATACA10.7633587786259541No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGCTGTCTCTT10.7633587786259541No Hit
GATTTTAATAACATGACATGAAGCCCCTAGACTTGATATAATCGTTATGCA10.7633587786259541No Hit
TGGTAAGACCGAACTTCAAATGCTTGCTTTTAAATCTTCAAAGATTAGACT10.7633587786259541No Hit
GAATTGAATGGAATCGTCATCGAATGAATTGAATGCAATCATCGAATGGTC10.7633587786259541No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG10.7633587786259541No Hit
GATAAATCCAATACAAACGAAGAACATAAATCAGAAAACCAAAATCTTTGC10.7633587786259541No Hit
GCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCAC10.7633587786259541No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGCTAGTCTGA10.7633587786259541Illumina PCR Primer Index 8 (96% over 27bp)
GTATGGAACTGAGAATGACTACTAGCACAAGGTTAAAGGCGTGTTTATACA10.7633587786259541No Hit
TAATTATCTCTGCTACAAAATATCCAAAGGAAACACCTGTCTCTTATACAC10.7633587786259541No Hit
TTCCATTTCAGTGTTGATTTCCAAAGTCTGATCATGATTCCTTCTGGGGTC10.7633587786259541No Hit
GAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGGAAACTTACCAGGTC10.7633587786259541No Hit
AAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAG10.7633587786259541No Hit
AACTATAACTGATTTAATGAGCCATTCGCAGTTTCACAGTCTGAATTCGTT10.7633587786259541No Hit
GATACCACTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG10.7633587786259541No Hit
GTGTATACATTTGTCAAAACTCATCAAACTGTACATTTAAAGCTGTCTCCT10.7633587786259541No Hit
ATTCTGTTTCAAGAATTCACTTCTCACTTCCTCAACACCTGTCTCTTATAC10.7633587786259541No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT10.7633587786259541No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGACTGTCTCTTATAC10.7633587786259541No Hit
GATGCAGCCATGTCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTT10.7633587786259541No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACCGACTACAGC10.7633587786259541RNA PCR Primer, Index 36 (96% over 29bp)
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTCTGTCTCTTATA10.7633587786259541No Hit
ACACAGATCGGTTCAAAGTCCAATGCAAAGCTACAGCTGTCTCTTATACAC10.7633587786259541No Hit
TACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7633587786259541No Hit
CCATACATGTAAAAAGCTAAGAGCAAATCATTACCTTTCTTCATTCTGATT10.7633587786259541No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCAGTC10.7633587786259541No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAAACGAGA10.7633587786259541Illumina PCR Primer Index 8 (96% over 27bp)
TCTTTATTTAGCATGCAGTTTGTGGCAATTCTATTGAGGAGGAAACAGGAT10.7633587786259541No Hit
GAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGA10.7633587786259541No Hit
GTACGGCGCGACCCCAGGTCGCGGGGCGGGATTACCCGCTGAGTTTAAGTA10.7633587786259541No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGATTTAGTACC10.7633587786259541No Hit
ACAATGTACAGAAGAAAGAAGAAAATTGTCGGATATAAGGGTCCTTGATCA10.7633587786259541No Hit
ATGAAAATAGTGATGTCGTATTGATACACATGCGTTACTTTATCAGGCGGC10.7633587786259541No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCAAAGAATAA10.7633587786259541Illumina PCR Primer Index 8 (96% over 27bp)
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATA10.7633587786259541No Hit
CTTATACCCATCTCCGAGCCCACGAGACTCTGGAACATCTCGTATGCCGTC10.7633587786259541No Hit
CACACATTGAGGACCAATTTGCAAGTGGACGTTTGTTGGCCTGTCTCTTAT10.7633587786259541No Hit
GATTAATTCCACCTCCTCTACACAACCATATGAAGCCCTTCCAATGGTAAA10.7633587786259541No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACCACGAAAA10.7633587786259541Illumina PCR Primer Index 8 (96% over 27bp)
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGA10.7633587786259541No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATACAC10.7633587786259541No Hit
GATAATTGCATTGAAACGAACAACTGGAAGTTTATCTCTTGTTGGGTGCTG10.7633587786259541No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATACACAAA10.7633587786259541Illumina PCR Primer Index 8 (96% over 27bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATAAGCTCCGTG10.7633587786259541Illumina PCR Primer Index 8 (96% over 27bp)
ATTCAAAAGTTGAATGTAACATTGTGTGCCAACTCTGTCTCTTATACACAT10.7633587786259541No Hit
TAAAACCACAGAGAAATAATCACACTTTTATGAGAGCATACTCCCTGTCTC10.7633587786259541No Hit
GTATCACGCAGAGTACGGGAACGGGGCTAAACCATGCACCGAAGTTGCGGC10.7633587786259541No Hit
GGATATCGGTTTCATTAAGTTGGACTAAATGCTCTTCCTTCAGAGGATTAT10.7633587786259541No Hit
CCTATAAACCATGCTAGGAGGACCTAAATTTATTCTGGTACAACAGAATTT10.7633587786259541No Hit
ATCAACGCAGAGTACGGGAAGCATGGTATCAACGCAGAGTACGGGGAAAAA10.7633587786259541No Hit
ACGCAGAGTACAGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG10.7633587786259541No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT10.7633587786259541No Hit
GAACTATACTACTAACACTCTTTCCTAAAATCCATTGTACAACTCCAACTT10.7633587786259541No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTGACTATCGCGTATGCCGT10.7633587786259541No Hit
AAATGCCAAGTATACCGCATTACTCGAACAAACTGAAACCAATGTCATCAT10.7633587786259541No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC10.7633587786259541No Hit
TCATTAATGGGATTGGAAATTTTAAGTGCCTTTGTGGACAGATTATCAACA10.7633587786259541No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCCTGTCTCTTATAC10.7633587786259541No Hit
CCGAAAGTAGTGGCGAGCGAAATCGGAAGAGCCTTCTAGTGATAGCACGAC10.7633587786259541No Hit
GGTGGTTATGGAGGGATTATTGTCAATAATCGCGCTATCATTCCGGTTCCT10.7633587786259541No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC10.7633587786259541No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACTCTTCCC10.7633587786259541RNA PCR Primer, Index 27 (96% over 28bp)
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTCTGTCTCTTATACA10.7633587786259541No Hit
GTGCAATACAGCGTAACAATTACACACTACCACTTAATGCTGTCTCTTATA10.7633587786259541No Hit
CTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCCCGTACCTGTCTCTTA10.7633587786259541No Hit
TATCAACGCAGAGTACGGGGGCATCAAAGCAGAGTACTTTCTGTCTCTTAA10.7633587786259541No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers