FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_74.35100000044c8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_74.35100000044c8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163973
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC131348.009855281052367No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT18091.103230409884554No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA8760.5342342946704641No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7940.48422606160770365No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7160.4366572545480048No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6750.41165313801662473No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5980.3646941874576912No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5900.35981533545156824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5000.30492825038268495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.2988296853750313No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4760.29029169436431607No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA4210.2567495868222207No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4070.24821159581150556No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA3650.22259762277936002No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG3510.21405963176864487No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3240.19759350624797986No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3170.19332451074262225No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3120.19027522823879542No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3030.18478651973190707No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2560.1561232641959347No Hit
ACTTAGATGTTTCAGTTCACTGCGTCTTCCTTTGATTAGCTATCTATTCAC2540.15490355119440394No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2240.13660785617144286No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2100.12806986516072769No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1680.10245589212858214No Hit
TTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCT1670.10184603562781677No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA1660.10123617912705139No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1660.10123617912705139No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC207.0155837E-445.03
AGGGACG207.0155837E-445.035
GAGGGAC207.0155837E-445.034
TGCCGTC14550.042.98969345
ATGCCGT16750.037.3432844
CGTATGC17350.036.18155741
GTATGCC17350.036.18155742
TGGACCA17250.036.13043631
ATCTCGT17400.036.07758337
TCGTATG17400.036.07758340
GACTCTG17350.036.05187226
CTCTGGA17300.036.02601228
AGGAGGG250.00210185536.032
AACATGC250.00210185536.08
CGCAGGA250.00210185536.040
TCTGGAC17250.036.029
ACCCAGT250.00210185536.07
GGAGGGA250.00210185536.033
CGAAGGA250.00210185536.029
CTCGTAT17450.035.97421339
Produced by FastQC (version 0.11.2)