FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_72.35100000044c47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_72.35100000044c47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165143
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC6190637.48629975233585No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT127167.699993339106108No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG115847.014526804042558No Hit
TTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTCT4930.2985291535214935No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAT207.0196844E-444.9948529
AGACCCG207.0196844E-444.9948525
TGCCGTC66150.044.3486745
GCCGTCT800.039.37049545
ATGCCGT78500.037.3715244
GACCCGC250.002103078235.9958826
GTTGTTA250.002103078235.995889
ACCCGCT250.002103078235.9958827
AGAGCCC250.002103078235.9958815
CCCGCTA250.002103078235.9958828
TATGCCG91350.032.1391843
GTATGCC91650.032.08307342
CGTATGC91700.031.99197841
CTCGTAT91400.031.97391339
TCGTATG91500.031.96355440
TCTCGTA91200.031.92068938
ATCTCGT91250.031.90319837
GATCTCG91150.031.88883636
GCTATGA91650.031.86214631
CGCTATG91650.031.837630
Produced by FastQC (version 0.11.2)