FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_71.35100000044c2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_71.35100000044c2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162548
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC6855342.174003986514755No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT2041512.55936707926274No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG31581.9428107389817162No Hit
TTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTCT10000.615202893914413No Hit
ATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTCTTC2060.1267317961463691Illumina PCR Primer Index 5 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGC207.0111407E-445.0055426
TGCCGTC71950.043.62941745
ATCCGAG250.002103706135.9933512
TATCTCG250.002103706135.9933536
ATGCCGT94650.033.14195644
TATGCCG97900.032.06472443
ATCTCGT97350.032.0609637
TCTCGTA97300.032.05430638
AGCCCAC97550.032.04136317
GCCCACG97500.032.03471418
CCCACGA97600.032.02494419
GATCTCG97200.032.00797736
CCACGAG97800.032.00547420
TCCGAGC98200.032.00274713
CTCGTAT97600.032.0018939
CTATGAT97400.031.99829132
CCGAGCC98150.031.99612814
GAGCCCA98150.031.99612816
ACGAGAC97900.031.99576622
TATGATC97550.031.99522833
Produced by FastQC (version 0.11.2)