FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_70.35100000044c03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_70.35100000044c03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136573
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC7686456.28052396886647No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT88916.510071536833781No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG36652.6835465282303237No Hit
TTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTCT4710.3448705088121371No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCATGATCTCGTATGCCGTC1470.10763474478850138No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATTTCGTATGCCGTC1410.1032414898991748No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC83100.044.26859745
ATGCCGT92700.039.63559744
CCCACGG356.208289E-638.57111719
GCCGTCT1350.038.33302345
TCCGAGC96700.038.13567713
TATGCCG96300.038.13052743
CACGAGA96200.038.1233921
CGAGACT96300.038.10716623
ACGAGAC96300.038.10716622
AGCCCAC96550.038.10170717
CCCACGA96200.038.119
GCCCACG96500.038.09813718
CCACGAG96150.038.09641320
CCGAGCC96800.038.09628314
AGACTCG96250.038.08020825
CTCCGAG96850.038.0766112
GTATGCC96200.038.0766142
CGTATGC96100.038.0694141
GAGCCCA96900.038.0569616
GAGACTC96550.038.05510324
Produced by FastQC (version 0.11.2)