FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_7.35100000044425.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_7.35100000044425.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15504
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC383124.709752321981423No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT2871.8511351909184726RNA PCR Primer, Index 44 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG1450.9352425180598555No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3750.042.045
CACGAGA3800.040.2631621
ACGAGAC3800.040.2631622
CGAGACC3800.040.2631623
GCCCACG3950.039.87341718
ATGCCGT3950.039.87341744
CCCACGA3850.039.7402619
AGCCCAC4000.039.37500417
GAGACCC3950.039.303824
CCACGAG3900.039.2307720
GACCCTA3850.039.15584626
AGACCCT3850.039.15584625
ATATCTC3800.039.0789535
GAGCCCA4150.039.03614416
CGAGCCC4150.039.03614415
TCCGAGC4150.039.03614413
TATCTCG3950.038.73417736
GAATATC3900.038.65384733
CTAGAAT3850.038.5714330
TAGAATA3850.038.5714331
Produced by FastQC (version 0.11.2)