H23LNBCXX_l01n01_bir_0113_pb_69.35100000044be8.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0113_pb_69.35100000044be8.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	318939
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC	82827	25.96954276523097	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT	13889	4.354751222020512	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG	5284	1.656743138970148	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT	1016	0.3185562129435409	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT	874	0.2740335926305657	No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC	782	0.2451879513010325	No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT	649	0.2034871872050768	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA	647	0.20286010804573917	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC	628	0.19690285603203123	No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT	622	0.19502161855401817	No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT	595	0.18655604990295951	No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT	572	0.1793446395705762	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT	566	0.17746340209256317	No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC	550	0.17244676881786172	No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA	525	0.16460827932614075	No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA	502	0.15739686899375743	No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC	476	0.1492448399223676	No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA	472	0.14799068160369225	No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA	433	0.1357626379966075	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT	432	0.13544909841693867	No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG	428	0.1341949400982633	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA	415	0.1301189255625684	No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA	411	0.12886476724389304	No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC	387	0.12133981733184088	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	379	0.11883150069449015	No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA	355	0.11130655078243804	No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATCT	345	0.10817115498574963	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC	345	0.10817115498574963	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG	343	0.10754407582641194	No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT	332	0.1040951404500547	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT	331	0.10378160087038588	No Hit
AAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTT	326	0.10221390297204166	No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCT	322	0.10095974465336632	No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA	319	0.1000191259143598	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCCGCT	20	7.0279033E-4	44.997025	27
TGCCGTC	8305	0.0	44.590664	45
ATGCCGT	9805	0.0	37.746098	44
CCTACAC	30	1.1353668E-4	37.515163	3
TCCTACA	30	1.1353668E-4	37.515163	2
CTCGCCA	30	1.13851645E-4	37.497517	28
GACCCGC	25	0.0021055155	35.997616	26
GCCGTCT	75	1.8189894E-12	35.997616	45
TATGCCG	10335	0.0	35.76686	43
GTATGCC	10350	0.0	35.69329	42
CGTATGC	10365	0.0	35.641636	41
GATCTCG	10340	0.0	35.619015	36
CTCGTAT	10350	0.0	35.60634	39
TATGATC	10295	0.0	35.599876	33
CTATGAT	10310	0.0	35.59173	32
ATCTCGT	10350	0.0	35.584602	37
ATGATCT	10340	0.0	35.575497	34
TCTCGTA	10360	0.0	35.571968	38
TCGTATG	10360	0.0	35.571968	40
CGCTATG	10325	0.0	35.56181	30

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content