FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_68.35100000044bce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_68.35100000044bce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103234
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC61045.912780673034078No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC5190.5027413449057481No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC4570.4426836119882985No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT3790.36712710928570047No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA3560.34484762771954974No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG2910.2818838754673848No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATACACATCTCCG2740.2654164325706647No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA2590.2508863358970882No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATACACATCTCC2410.23345021988879633No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2370.22957552744250925No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG2370.22957552744250925No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG2340.22666950810779396No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2340.22666950810779396No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2340.22666950810779396No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCT2230.21601410388050446No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATA2210.21407675765736095No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2180.21117073832264563No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATA2120.20535869965321502No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC2110.20439002654164326No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCT2020.19567196853749733No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC1970.1908286029796385No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.18889125675649493No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTC1830.17726717941763373No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCTC1780.17242381385977487No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG1740.16854912141348782No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1680.16273708274405718No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT1660.16079973652091364No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGCTGTCTCTTAT1550.15014433229362417No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1500.14530096673576534No Hit
CTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTAT1480.1433636205126218No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1460.14142627428947827No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCT1430.13852025495476297No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC1420.1375515818431912No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCT1420.1375515818431912No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA1350.1307708700621888No Hit
CTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTT1310.12689617761590175No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCCTGTCTCTTATACACA1280.12399015828118644No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTC1280.12399015828118644No Hit
GTATCAACGCAGAGTACGGGCAGCTGTCTCTTATACACATCTCCGAGCCCA1270.12302148516961467No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.12205281205804289No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTC1200.11624077338861227No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT1180.11430342716546875No Hit
GCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACA1160.1123660809423252No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1150.11139740783075343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.11042873471918166No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA1140.11042873471918166No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGCTGTCTCTT1090.10558536916132281No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.10364802293817929No Hit
GGTATCAACGCAGAGTACGGGCAGCTGTCTCTTATACACATCTCCGAGCCC1060.10267934982660751No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT1060.10267934982660751No Hit
GCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC1060.10267934982660751No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCCTGTCTCTTATACACATCTCC1050.10171067671503574No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGCTGTCTCTTATA1040.10074200360346397No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7150.043.4438945
ATGCCGT7800.039.82356644
GCTATGA8150.038.94190231
CTATGAT8050.038.86642532
ATGATCT8050.038.84758434
CGCTATG8200.038.70445330
TCGCTAT8200.038.70445329
TATGATC8100.038.62650733
ACTCGCT8250.038.4698827
AGACTCG8250.038.4698825
GACTCGC8250.038.4698826
CTCGCTA8250.038.4698828
GTATGCC8150.038.38953842
TCTCGTA8150.038.37092638
CGTATGC8150.038.37092641
TCGTATG8150.038.37092640
CTCGTAT8150.038.37092639
CACGAGA8350.038.00916321
TGATCTC8250.037.9242135
TATGCCG8250.037.9242143
Produced by FastQC (version 0.11.2)