FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_65.35100000044b61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_65.35100000044b61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC67.4074074074074066No Hit
CTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCC11.2345679012345678No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGG11.2345679012345678No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCC11.2345679012345678No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCTTAGATCTCGTATGCCGTC11.2345679012345678No Hit
GTACAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA11.2345679012345678No Hit
TCTTATACACATCTCCGAGCCCACGAACTGTTATACATCTCGTATGCCGTC11.2345679012345678RNA PCR Primer, Index 39 (95% over 21bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACCCCCCCGCC11.2345679012345678Illumina PCR Primer Index 8 (96% over 27bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGTATGCCGTC11.2345679012345678Illumina PCR Primer Index 8 (96% over 27bp)
AAAGAGGGAGGGAGAGATCTGAGATCAGAGAGTCTGTCTCTTATACACATC11.2345679012345678No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAGTACT11.2345679012345678No Hit
AATATGCGTAGTATTAGGGGCATTAAAAATCTGCATCTAATGTAAACTTCA11.2345679012345678No Hit
ATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCA11.2345679012345678No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGA11.2345679012345678No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACCGAGCCCC11.2345679012345678Illumina PCR Primer Index 8 (96% over 27bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACCCCGTACTCT11.2345679012345678No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTG11.2345679012345678No Hit
GAGTTACATGTGTGCGTGATTGTCTAAAGCTATTTACTTTCTTGGGAGCCT11.2345679012345678No Hit
AGGCAAAGGGAAGGATTTTGAAAAACTAGCCAAGAACATGACAACCTCTAA11.2345679012345678No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAATCCGTG11.2345679012345678RNA PCR Primer, Index 27 (96% over 28bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACAAAAATACGCC11.2345679012345678RNA PCR Primer, Index 27 (96% over 28bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACCAAA11.2345679012345678RNA PCR Primer, Index 27 (96% over 28bp)
GCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT11.2345679012345678No Hit
ATATAATCCATTAGACAAGAAGACAAAGGTTAGATTTTCTTCAGAAGTTTG11.2345679012345678No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA11.2345679012345678No Hit
TATCCGTTCTTGTCCGTCTTTGTCTCCGATATTGAGTTCTTCTTGTTCTTG11.2345679012345678No Hit
CGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATGCA11.2345679012345678No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTATGCCGCC11.2345679012345678Illumina PCR Primer Index 8 (96% over 27bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATAACTTCCCTT11.2345679012345678RNA PCR Primer, Index 27 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGACTGATTACAATCTCGTATGCCGTCTT11.2345679012345678RNA PCR Primer, Index 22 (95% over 21bp)
AAGGAGATGAGAAGGATGAGAAAAGTGAGTACTTTTCCTTCATAAAAGCCA11.2345679012345678No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAACAAA11.2345679012345678Illumina PCR Primer Index 8 (96% over 27bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAACCCACG11.2345679012345678Illumina PCR Primer Index 8 (96% over 27bp)
GGCGATTTAAGAGTCTCTGCTGTTTCGTTTCCAGAAGATGCTTTGTGAATT11.2345679012345678No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG11.2345679012345678No Hit
TTGGCATTCCCTTACAAAACCTGGTGGAAAACTCTAAATTACAACTTCCAG11.2345679012345678No Hit
CCAATATACTGATTACCAACTGACATTGGTGTCAAAAGCAGTCTAATGCTT11.2345679012345678No Hit
GCTCTGATGCCGCCGTGTTTCGGCTGTCAGCGCAGGGGCTGTCTCTATACA11.2345679012345678No Hit
CATTTGAGGATATGAACTAGGTTGGTACTTGGGCTGTCTCTTATACACATC11.2345679012345678No Hit
AATGATAACTAATGACATCCAGTGTCTCCAAAATTGTTTCCTTGTACTGAT11.2345679012345678No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAAC11.2345679012345678No Hit
CAGTAGCTAGTATTTCTGTTATACAACTGGTTGTGAACAGGAAGAACAAAA11.2345679012345678No Hit
TCAGTACAGACATTTCAATTTGTACACAAATCTTAACATACGTAACGAAAT11.2345679012345678No Hit
CTTATACACATCTCCGAGCCCACGGACTGCTCACAAACCCGTTATCCGGCC11.2345679012345678No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT11.2345679012345678No Hit
GTATATTATTGTCATAGCCTGATAGCCAGTTTTCCTGTCTCTAGCCTTTAG11.2345679012345678No Hit
GGCATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGC11.2345679012345678No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAACCT11.2345679012345678No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAAGACGA11.2345679012345678RNA PCR Primer, Index 27 (96% over 28bp)
TCTGTGATAAGCGTCGGTAAGGTGATATGAACCTGTCTCTTATACACATCT11.2345679012345678No Hit
TCTTATACACATCTCCGAGCCCAGAGACGAGAGTTGATCTCGTATGCCGTC11.2345679012345678No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGCCAA11.2345679012345678Illumina PCR Primer Index 8 (96% over 27bp)
GTATCAACGCAGAGTACGGGGAGCGAACGGGGAGCAGCCCAGAGCCTGAAT11.2345679012345678No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT11.2345679012345678No Hit
TGCCCACGAAGGCGGCTGGCGGACGGCGACGACGATCGTTTTCATGGTGGC11.2345679012345678No Hit
TCTTATACACATCTCCGAGCCCACGAGCGTCCTAGTATCTCGTATGCCGTC11.2345679012345678No Hit
CTTTTTAACTGCAACAACTTTAATATACGCTATTGGAGCTCTGTCTCTTAT11.2345679012345678No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAAAAA11.2345679012345678Illumina PCR Primer Index 8 (96% over 27bp)
GGTATCAACGCAGAGTACGGGTGCCTGTCTCTTATACACATCTCCGAGCCC11.2345679012345678No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA11.2345679012345678No Hit
CTAAGTAACCTGTGGATAAATATTTGGACACTATGAAAATATTCTTTCCAA11.2345679012345678No Hit
ATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAAGTACGGGAAGCA11.2345679012345678No Hit
ATTTAGATGAAAAACTGCATTCCATATAAATTGTGACATTGTGTCTGTCTC11.2345679012345678No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGACAGA11.2345679012345678RNA PCR Primer, Index 27 (96% over 28bp)
CTTATACACATCTCCGAGCCCAGAGACGTCTTAGTATCTCGTATGCCGTCT11.2345679012345678No Hit
TTTTTTTTTTTTTTGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCA11.2345679012345678No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTAC11.2345679012345678No Hit
GGTATCAACGCAGAGTACGGGAACCTGTCTCTTATACACATCTCCGAGCCC11.2345679012345678No Hit
GTGTAGTGTAAACATGTTGTTACACAATGTAGTAAATCCCAGTTATAACAC11.2345679012345678No Hit
GACTTGCTTAGATGCCAATAGACATTATGATCCATGATCTGTCTCTTATAC11.2345679012345678No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT11.2345679012345678No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTTATAC11.2345679012345678No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCAATAATCTCGTATGCCGTC11.2345679012345678No Hit
GCTTCCCGTACTCTGCGTTGATACCACCCAAAAAAAAAAAAAAAAAAAAAA11.2345679012345678No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATATTATGATGG11.2345679012345678Illumina PCR Primer Index 8 (96% over 27bp)
CCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCAA11.2345679012345678No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC50.045.012
AATCTCG50.045.06
ATCTCGT50.045.07
TAAATCT50.045.04
AAATCTC50.045.05
TGCCGTC50.045.015
AAACAAA50.045.045
TATGCCG50.045.013
TATAAAT50.045.02
GAAAAAA50.045.030
TTCTGCT50.045.022
CCGTCTT50.045.017
TCTGCTT50.045.023
TCTCGTA50.045.08
ATAAATC50.045.03
TGCTTGA50.045.025
ATGCCGT50.045.014
GTCTTCT50.045.019
CGTATGC50.045.011
GCTTGAA50.045.026