FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_64.35100000044b48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_64.35100000044b48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22456
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC995144.31332383327396RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT4341.9326683291770574No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG4301.9148557178482364No Hit
TTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCT400.17812611328820804RNA PCR Primer, Index 39 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT302.0716234E-645.00000445
TGCCGTC11000.043.56818445
ATGCCGT11400.042.03947444
TCCGAGC11700.041.34615713
GCCCACG11650.041.3304718
TATGCCG11600.041.31465543
GTATGCC11550.041.29870242
CACGAGA11750.041.1702121
ACGAGAC11750.041.1702122
GTTATAC11750.041.1702130
ACTGTTA11700.041.15384727
AGCCCAC11700.041.15384717
CCCACGA11700.041.15384719
TGTTATA11700.041.15384729
GACTGTT11700.041.15384726
CGAGCCC11700.041.15384715
CCGAGCC11700.041.15384714
CTGTTAT11700.041.15384728
TCGTATG11650.041.1373440
CTCGTAT11550.041.10389739
Produced by FastQC (version 0.11.2)