Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0113_pb_63.35100000044b2e.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 130735 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC | 42346 | 32.39071403985161 | RNA PCR Primer, Index 39 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT | 5723 | 4.377557654797873 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG | 4853 | 3.712089341033388 | No Hit |
TTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCT | 215 | 0.16445481317168317 | RNA PCR Primer, Index 39 (100% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 4550 | 0.0 | 44.056694 | 45 |
ATGCCGT | 5175 | 0.0 | 38.692364 | 44 |
TATGCCG | 5660 | 0.0 | 35.37685 | 43 |
GTTATAC | 5645 | 0.0 | 35.32495 | 30 |
GACTGTT | 5685 | 0.0 | 35.31394 | 26 |
ACGAGAC | 5715 | 0.0 | 35.31196 | 22 |
AGCCCAC | 5730 | 0.0 | 35.298046 | 17 |
TATACAT | 5650 | 0.0 | 35.293686 | 32 |
CTCGTAT | 5630 | 0.0 | 35.285633 | 39 |
TACATCT | 5630 | 0.0 | 35.285633 | 34 |
ACTGTTA | 5690 | 0.0 | 35.282906 | 27 |
CACGAGA | 5720 | 0.0 | 35.281094 | 21 |
CCCACGA | 5720 | 0.0 | 35.281094 | 19 |
CCACGAG | 5720 | 0.0 | 35.281094 | 20 |
TCTCGTA | 5625 | 0.0 | 35.277 | 38 |
ATACATC | 5635 | 0.0 | 35.267815 | 33 |
GAGACTG | 5700 | 0.0 | 35.26049 | 24 |
GCCCACG | 5730 | 0.0 | 35.25878 | 18 |
ATCTCGT | 5635 | 0.0 | 35.254322 | 37 |
CGTATGC | 5655 | 0.0 | 35.24899 | 41 |