FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_63.35100000044b2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_63.35100000044b2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130735
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC4234632.39071403985161RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT57234.377557654797873No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG48533.712089341033388No Hit
TTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCT2150.16445481317168317RNA PCR Primer, Index 39 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC45500.044.05669445
ATGCCGT51750.038.69236444
TATGCCG56600.035.3768543
GTTATAC56450.035.3249530
GACTGTT56850.035.3139426
ACGAGAC57150.035.3119622
AGCCCAC57300.035.29804617
TATACAT56500.035.29368632
CTCGTAT56300.035.28563339
TACATCT56300.035.28563334
ACTGTTA56900.035.28290627
CACGAGA57200.035.28109421
CCCACGA57200.035.28109419
CCACGAG57200.035.28109420
TCTCGTA56250.035.27738
ATACATC56350.035.26781533
GAGACTG57000.035.2604924
GCCCACG57300.035.2587818
ATCTCGT56350.035.25432237
CGTATGC56550.035.2489941