FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_62.35100000044b04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_62.35100000044b04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154481
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC7060245.702707776360846RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT104886.789184430447757No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG62404.039331697749238No Hit
TTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCT4150.26864145105223297RNA PCR Primer, Index 39 (100% over 21bp)
ATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCTTC1620.10486726523002829RNA PCR Primer, Index 39 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAAA207.0158066E-444.9980624
TGCCGTC72950.044.3503845
ATGCCGT84900.038.10789544
CTTTATA250.002098584336.01012
CTGCTTG250.002101923835.99844424
CATCTCG90900.035.29550636
ATACATC91100.035.2921133
TATGCCG91500.035.28536243
CTCGTAT90950.035.25136639
TCTCGTA90900.035.24600238
TACATCT91100.035.24271834
CGTATGC91500.035.23618341
TCGTATG91250.035.23409340
ATCTCGT91000.035.23199537
CACGAGA92000.035.2158721
CCACGAG91950.035.21055220
CCCACGA91900.035.20522719
GTATGCC91650.035.20306442
TATACAT91650.035.20306432
ACGAGAC92050.035.19674322