H23LNBCXX_l01n01_bir_0113_pb_61.35100000044adc.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0113_pb_61.35100000044adc.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	350684
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC	8430	2.403873572789178	RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT	1520	0.4334386513214176	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG	987	0.2814499663514731	No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC	503	0.14343397474649544	No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA	451	0.12860581035918375	No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC	423	0.12062141415063134	No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA	396	0.11292217494952722	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	860	0.0	43.688953	45
TAGTATA	25	0.002101385	36.013012	4
ATGCCGT	1045	0.0	35.73925	44
TATGCCG	1145	0.0	32.42142	43
CGTATGC	1155	0.0	32.140717	41
CTCGTAT	1150	0.0	32.084824	39
TCGTATG	1155	0.0	31.945925	40
CATCTCG	1170	0.0	31.728659	36
TATACAT	1190	0.0	31.573528	32
TCTCGTA	1175	0.0	31.402164	38
TGTTATA	1195	0.0	31.253149	29
GTATGCC	1200	0.0	30.935442	42
GTTATAC	1220	0.0	30.79713	30
ATCTCGT	1200	0.0	30.747953	37
TACATCT	1225	0.0	30.487766	34
ACTGTTA	1245	0.0	30.178715	27
ATACATC	1255	0.0	30.117517	33
GGGTGGC	30	0.0051441123	29.998003	18
CTGTTAT	1255	0.0	29.938248	28
CTAACGC	55	4.1424228E-6	28.646711	3