FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_6.3510000004440b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_6.3510000004440b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24546
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC1246750.79035280697466No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT6252.5462397131915586RNA PCR Primer, Index 44 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG5082.069583638882099No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT580.23629104538417664RNA PCR Primer, Index 44 (95% over 23bp)
CTTATACACATCTCCGAGCCACGAGACCCTAGAATATCTCGTATGCCGTCT330.1344414568565143RNA PCR Primer, Index 44 (95% over 23bp)
CTTATACACATCTCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT260.10592357206876885RNA PCR Primer, Index 44 (95% over 23bp)
CTTATACACATCCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT250.10184958852766234RNA PCR Primer, Index 37 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCGTATGCCGTCTT250.10184958852766234No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC13650.042.52747345
CTCCGAG14500.041.2758612
ATCTCCG14650.041.16040810
TCTCCGA14600.041.14726311
TCCGAGC14450.041.10726513
CCGAGCC14500.040.96551514
AGCCCAC14400.040.937517
CCCACGA14350.040.92334719
GCCCACG14300.040.90909218
ATGCCGT14200.040.88028344
GAGCCCA14550.040.8247416
CGAGCCC14450.040.7958515
CATCTCC14800.040.7432449
ACGAGAC14600.040.37671322
CCACGAG14700.040.25510420
CACGAGA14700.040.25510421
TATCTCG14500.040.0344836
ATATCTC14500.040.0344835
AATATCT14450.040.01730334
GACCCTA14800.039.9831126