Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0113_pb_6.3510000004440b.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 24546 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC | 12467 | 50.79035280697466 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT | 625 | 2.5462397131915586 | RNA PCR Primer, Index 44 (95% over 21bp) |
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG | 508 | 2.069583638882099 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT | 58 | 0.23629104538417664 | RNA PCR Primer, Index 44 (95% over 23bp) |
CTTATACACATCTCCGAGCCACGAGACCCTAGAATATCTCGTATGCCGTCT | 33 | 0.1344414568565143 | RNA PCR Primer, Index 44 (95% over 23bp) |
CTTATACACATCTCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT | 26 | 0.10592357206876885 | RNA PCR Primer, Index 44 (95% over 23bp) |
CTTATACACATCCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT | 25 | 0.10184958852766234 | RNA PCR Primer, Index 37 (95% over 21bp) |
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCGTATGCCGTCTT | 25 | 0.10184958852766234 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 1365 | 0.0 | 42.527473 | 45 |
CTCCGAG | 1450 | 0.0 | 41.27586 | 12 |
ATCTCCG | 1465 | 0.0 | 41.160408 | 10 |
TCTCCGA | 1460 | 0.0 | 41.147263 | 11 |
TCCGAGC | 1445 | 0.0 | 41.107265 | 13 |
CCGAGCC | 1450 | 0.0 | 40.965515 | 14 |
AGCCCAC | 1440 | 0.0 | 40.9375 | 17 |
CCCACGA | 1435 | 0.0 | 40.923347 | 19 |
GCCCACG | 1430 | 0.0 | 40.909092 | 18 |
ATGCCGT | 1420 | 0.0 | 40.880283 | 44 |
GAGCCCA | 1455 | 0.0 | 40.82474 | 16 |
CGAGCCC | 1445 | 0.0 | 40.79585 | 15 |
CATCTCC | 1480 | 0.0 | 40.743244 | 9 |
ACGAGAC | 1460 | 0.0 | 40.376713 | 22 |
CCACGAG | 1470 | 0.0 | 40.255104 | 20 |
CACGAGA | 1470 | 0.0 | 40.255104 | 21 |
TATCTCG | 1450 | 0.0 | 40.03448 | 36 |
ATATCTC | 1450 | 0.0 | 40.03448 | 35 |
AATATCT | 1445 | 0.0 | 40.017303 | 34 |
GACCCTA | 1480 | 0.0 | 39.98311 | 26 |