H23LNBCXX_l01n01_bir_0113_pb_57.35100000044a56.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0113_pb_57.35100000044a56.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	168380
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC	26677	15.843330561824445	RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT	11130	6.610048699370472	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG	2691	1.5981708041335074	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	2672	1.5868868036583919	No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA	1439	0.8546145622995605	No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC	1188	0.7055469770756622	No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA	1057	0.627746763273548	No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA	863	0.512531179474997	No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC	553	0.32842380330205484	No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA	551	0.3272360137783585	No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA	531	0.31535811854139445	No Hit
TTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCT	466	0.2767549590212614	RNA PCR Primer, Index 39 (100% over 21bp)
AAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG	458	0.27200380092647586	No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC	438	0.2601259056895118	No Hit
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG	426	0.2529991685473334	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA	415	0.24646632616700323	No Hit
AAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA	413	0.24527853664330682	No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG	400	0.2375579047392802	No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC	361	0.21439600902720038	No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC	305	0.18113790236370114	No Hit
AAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCC	286	0.16985390188858535	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT	255	0.15144316427129112	No Hit
AAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCC	238	0.14134695331987174	No Hit
GGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACG	221	0.1312507423684523	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC	214	0.1270934790355149	No Hit
AAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCC	212	0.1259056895118185	No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA	201	0.1193728471314883	No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT	201	0.1193728471314883	No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC	192	0.11402779427485449	No Hit
AAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACGAG	187	0.1110583204656135	No Hit
AAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACG	182	0.10808884665637249	No Hit
GTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCA	180	0.10690105713267609	No Hit
GGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTCCGAGCCC	176	0.1045254780852833	No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT	175	0.10393158332343509	No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG	169	0.10036821475234588	No Hit
GGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAGCC	169	0.10036821475234588	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	2895	0.0	42.678818	45
GCCGTCT	150	0.0	34.508427	45
ATGCCGT	4035	0.0	30.620861	44
GTGTTAA	30	0.005128801	30.007326	1
CTGTGTC	30	0.0051362594	29.998417	13
TATGCCG	4240	0.0	29.184778	43
GTATGCC	4245	0.0	29.097404	42
ATCTCGT	4235	0.0	29.05986	37
TGTTATA	4260	0.0	29.04776	29
CGTATGC	4245	0.0	29.044403	41
CTGTTAT	4265	0.0	29.013708	28
CATCTCG	4235	0.0	29.006733	36
GTTATAC	4265	0.0	28.960957	30
TCTCGTA	4250	0.0	28.957296	38
TCGTATG	4260	0.0	28.942133	40
ATACATC	4260	0.0	28.942133	33
ACTGTTA	4280	0.0	28.912025	27
TACATCT	4265	0.0	28.908205	34
GACTGTT	4290	0.0	28.897074	26
TATACAT	4275	0.0	28.893211	32

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content