FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_56.35100000044a3c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_56.35100000044a3c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148928
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC2776018.639879673399225No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT78205.250859475719811No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCG12150.8158304684142673No Hit
TTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTCT4930.33103244520842284No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCAA207.0137565E-444.99966413
TGCCGTC30700.043.2407245
CGTCTTC250.002101312435.99973345
GCCGTCT1250.034.19974545
ATGCCGT39250.033.7640844
ACGAGAC40650.032.6565822
GCCCACG40750.032.6316618
CACGAGA40700.032.61646721
CGAGACT40700.032.61646723
ACTACCG40650.032.60123427
CCACGAG40650.032.60123420
TATGCCG40600.032.58596443
CTACCGC40600.032.58596428
TACCGCT40600.032.58596429
CGTATGC40750.032.57644341
GATCTCG40350.032.56481636
CTCGTAT40500.032.55531339
ACCGCTG40450.032.53993230
CCCACGA40800.032.53652219
AGCCCAC40900.032.51198217
Produced by FastQC (version 0.11.2)