FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_55.35100000044a12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_55.35100000044a12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174610
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC4942128.303648130118546No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT1957611.211270832140197No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCG18721.0721035450432392No Hit
TTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTCT10520.6024855392016494No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCG2600.14890327014489432No Hit
ATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTCTTC2040.11683179657522479TruSeq Adapter, Index 10 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC54450.042.72400745
GCCGTCT2700.036.66386845
TATCTCG250.00210301635.99725336
ATGCCGT75050.030.96699144
TGAACAG300.0051229930.0148985
AGTATGC300.00513736829.9977141
TAGTATG300.00513736829.9977140
TCCGAGC78000.029.88233213
CCGAGCC78000.029.88233214
CCCACGA77850.029.8821119
CCACGAG77850.029.8821120
CGAGCCC77950.029.87263915
TCTCGTA76900.029.8709338
GCCCACG77900.029.8629318
GAGACTA77750.029.86267324
CTCGTAT77150.029.8616239
ATCTCGT77000.029.86135737
CACGAGA78000.029.85348921
CGAGACT77850.029.85321223
TATGCCG77900.029.8340543
Produced by FastQC (version 0.11.2)