FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_54.351000000449f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_54.351000000449f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24166
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC396016.38665894231565No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT1890.7820905404287015No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCG1550.6413970040552843No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4350.043.44827345
ATGCCGT4550.041.5384644
TATGCCG4850.038.96907443
GCCCACG4900.038.5714318
CCCACGA4900.038.5714319
CGCTGAT4850.038.50515732
CACGAGA4850.038.50515721
ATCTCGT4800.038.43749637
TCTCGTA4800.038.43749638
CGAGACT4750.038.3684223
GAGACTA4750.038.3684224
AGCCCAC5000.038.2517
CGTATGC4950.038.18181641
TCGTATG4950.038.18181640
CCACGAG4900.038.11224420
CCGCTGA4900.038.11224431
CTCGTAT4900.038.11224439
TGATCTC4850.038.04123735
CTGATCT4850.038.04123734
AGACTAC4750.037.89473725