FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_53.351000000449dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_53.351000000449dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76905
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC3340.43430206098433133No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG1340.17424094662245626No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA970.1261296404655094No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG206.9927546E-444.999357
TCGCGCG206.9927546E-444.999359
TACCGCT501.0531949E-940.49941329
CGTATGC451.8886567E-839.9994241
TATGCCG451.8886567E-839.9994243
TCGTATG451.8886567E-839.9994240
CCCACGA552.6793714E-936.8176519
CTACCGC552.6793714E-936.8176528
GTCGCGC250.002095080235.9994778
CTCGTAT504.779031E-835.99947739
ATAGGTC250.002095080235.9994774
TAGGTCG250.002095080235.9994775
AAGCGCC250.002095080235.99947719
TCTCGTA504.779031E-835.99947738
GCTAGTT250.002095080235.99947739
TATAGGT250.002095080235.9994773
ATGCCGT504.779031E-835.99947744
CTGATCT504.779031E-835.99947734
GGCTAGT250.002095080235.99947738
AGGTCGC250.002095080235.9994776
Produced by FastQC (version 0.11.2)