FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_52.351000000449b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_52.351000000449b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358317
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC318358.884591018567358No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT94462.6362131855312474No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC22780.6357499086004851No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC19200.5358383777493114No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA13730.38318025658843985No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCG12440.34717861558340796No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA11990.3346199036049085No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA10210.28494322066773276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA9370.2615002916412004No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA9270.2587094667570894No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA8180.22828947552027953No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT7780.21712617598383552No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC7260.20261388658645835No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC7070.19731131930664747No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA6620.18475260732814797No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG6000.1674494930466598No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT5550.15489078106816032No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA5340.14903004881152723No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5250.14651830641582733No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT5200.14512289397377184No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC4780.13340142946050565No Hit
TTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTCT4670.13033152208798354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG4630.12921519213433913No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT4600.12837794466910585No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC4400.12279629490088385No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC4270.11916822255153955No Hit
AAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA4210.11749372762107296No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC3840.10716767554986228No Hit
AAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCC3810.10633042808462898No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC3810.10633042808462898No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT3800.10605134559621787No Hit
GGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAGCC3770.10521409813098456No Hit
TATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT3670.10242327324687357No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC3640.10158602578164028No Hit
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG3640.10158602578164028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC33700.042.52677545
ATCGTCA250.002105882235.99754313
CCTTGTC401.5583099E-533.74769624
TGTCGAA551.12469934E-732.72503733
ATGCCGT44600.032.13345744
GCCGTCT1650.031.36149845
TATGCCG45950.031.18938343
CTACCGC46000.031.15548328
CGCTGAT45800.031.14416532
CCGCTGA46050.031.12165531
CGAGACT46200.031.11800623
ACTACCG46000.031.10657327
ACCGCTG46150.031.1029730
TACCGCT46100.031.08790229
GCTGATC46000.031.05766533
GATCTCG45450.031.03748736
ACGAGAC46400.031.03236422
GTATGCC45900.031.02729442
GACTACC46150.031.00546826
GAGACTA46300.031.00220524
Produced by FastQC (version 0.11.2)