FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_51.3510000004499a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_51.3510000004499a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC53.546099290780142No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT21.4184397163120568No Hit
AACTAAAACAGAGGTAACACAACACAAAGTACAAGAACAAGTAGACATTAA10.7092198581560284No Hit
TTATTGATGACCGTGTCACATTCACTTTAACGAGTTTAACACCTCGTGGTT10.7092198581560284No Hit
GTGTTGTAGTACGCTTGAGGAGCAACAGCAGCAGCCGGTTGCTGCACCGGT10.7092198581560284No Hit
GATATATTTCTTTAGTCTTTGTGAGTGTGAGAAGACCAAAAATAGAATCGT10.7092198581560284No Hit
AAGCTGCCATGGCCCTGGAGAAAAAGCTGAACCAGGCCCTTTTGGATCTTC10.7092198581560284No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCTC10.7092198581560284No Hit
GTATCAACGCAGAGTACGGGCATGGTATCAACTCAGAGTACTTTTTCTGTC10.7092198581560284No Hit
CATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATACTAAAACT10.7092198581560284No Hit
AAAACGATTGGCTCTGAGGGCTGGGCTCGGGGGTCCCAGTTCCGAACCCGT10.7092198581560284No Hit
GTACAAAGAGAATCATCCAAATCAACGTCCCATTTCAAAACCTTCCCTTCT10.7092198581560284No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACACTCCTAC10.7092198581560284RNA PCR Primer, Index 27 (96% over 28bp)
GAATCCAATCAACGCAATGTCTACATAGCCAAAGTCATCGCCACTAAAGTA10.7092198581560284No Hit
GGAGATTATTGTTCTCTTCACACTTATATACTGTCTCTTATACACATCTCC10.7092198581560284No Hit
TACCAGTTGTGTTCGAAATGATATTAAGAAAGAAGCCGCGGGAATTTCCAG10.7092198581560284No Hit
GTCTCAAAGAAATTGAGGTCAGGCTTCCTTCTTACAGAATTATTTTTTTCT10.7092198581560284No Hit
CTGCTACAACTTTGAGCAAACCTTGAAGCACAGCTGCGAGATGAGCACTTG10.7092198581560284No Hit
GGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAA10.7092198581560284No Hit
GAATATAGAGTTGTACTTACATGTTGTGCCTGCATAATGCCTGTCTCTTAT10.7092198581560284No Hit
GGTAAGTATTTACCATTAACTGGAGCAAATATTAGACTGTCTCTTATACAC10.7092198581560284No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTCTGATCTCGTATGCCGTC10.7092198581560284No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATAGAACTC10.7092198581560284Illumina PCR Primer Index 8 (96% over 27bp)
TGTCCTTCTTGTTCTTCCTCTTGAACTCTTGAACAACTGTCTCTTATACAC10.7092198581560284No Hit
ATTCTATACGACGAGTTCATCTTCAAGATAACTGTATTCACTGTCTCTTAT10.7092198581560284No Hit
TACCAATACGGTAATCAATGCTGAGAATGCGAGTATGAAGACAATGAACAC10.7092198581560284No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTAAAAAAAAAAAAAAGTACTCT10.7092198581560284No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATTTAGACC10.7092198581560284RNA PCR Primer, Index 27 (96% over 28bp)
GTCTTAGAGCTTGGAGATCTTCCAATGATTGCTTTCTTTGCCTTAACAAGA10.7092198581560284No Hit
GGACTAGATGGGATCTACTGTATGTTTCTAGGGAGATTGAATAATCTCTGT10.7092198581560284No Hit
GTGTATTATTTCACTCTTGTGGTTTTTTGGCCTCACTCTTTGTGGACCACT10.7092198581560284No Hit
ACCTACTGTATGCTTCATCTAACATTGTTTGTATATCTAGATCTGTCTCTT10.7092198581560284No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATAATGAATAG10.7092198581560284RNA PCR Primer, Index 27 (96% over 28bp)
GTACGGGGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATTA10.7092198581560284No Hit
TCTTAATTGCAACATCTTCTACGAATTAATCAAAAGCCAAAAACATATACC10.7092198581560284No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAATAAAACTGTCACTTA10.7092198581560284Illumina PCR Primer Index 8 (96% over 27bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTC10.7092198581560284No Hit
TATACGAGAGAGCTAAAACAAATGGATTCCAAATCCAGCAAGTACAAAAAT10.7092198581560284No Hit
AAAAGTACTCTGCGTTGATACCCCGTACCTGTCTCTTATACACATCTCCGA10.7092198581560284No Hit
GTAAAGAAGAATCCTTTCTTCTTCTTCCTCGTAAAATCAGAAGATAAGCAT10.7092198581560284No Hit
GTCACATTCCACAGGAAGAAGAATCTGCAGTACTATGAGATCTGTCTCATA10.7092198581560284No Hit
ATTTGTAGTCATGTACAGAACATGATCAAGGGTGTTACACTGGGCTTCCGT10.7092198581560284No Hit
AGTTAAGGTTGTTCTTGAAATATGATCAAAGAAATGGACACTGACTGTCTC10.7092198581560284No Hit
GCTCGGGGGTCCCAGTTCCGAACCCGTCGGCTGTCAGCGGACTGCTCGAGC10.7092198581560284No Hit
TTTAGGTATTATACGAACTGAAGAAAGAGATGTTACGTAGGCTGTCTCTTA10.7092198581560284No Hit
ATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTACTGTCTC10.7092198581560284No Hit
GAAGTGGAGAGTGAATTTGTATGACTAAAGGATAATCTGTACATGGGAAGT10.7092198581560284No Hit
CTATTGGGAAAACCTTCAGGAAGAAGGAATTGAGTGAGAAGGTTTCTACAT10.7092198581560284No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCGTATACACATCTCTGAGCCCA10.7092198581560284No Hit
GCATCAGCGTGCTCCGGGCGTCGGCCTGTGGGCTCCCCATTCGACCCGTCT10.7092198581560284No Hit
ATATTGGTGATCAAATACAGATACTAAGCAACTCAACATACAAGAGCTGTC10.7092198581560284No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGTAACATA10.7092198581560284Illumina PCR Primer Index 8 (96% over 27bp)
GTTCTTATCATCTTAGAGAGAGGTTAAAGACAGGAGAACCGTGCATCTATC10.7092198581560284No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG10.7092198581560284No Hit
TTTTTCCTTCTGTCACAAAATCCTGAAAAAATTAGGAACTGTCTATTATAC10.7092198581560284No Hit
ATGTTTCAGATTGGGAAAATGAGGTACGTTAGTGTTCGCGATTTTAAAGGC10.7092198581560284No Hit
TCATTAGCCTCTAGATATAAGTTTCCTCCTCCTGTCTCTTATACACATCTC10.7092198581560284No Hit
GTACTATACTTCACAACAATCCTAATCCTAATACCAACCTGTCTCTTATAC10.7092198581560284No Hit
CAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGT10.7092198581560284No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT10.7092198581560284No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAGCAGATCTCGTATGCCGTC10.7092198581560284No Hit
CCACAGAACGTACCTGGTTTGGGATCAGAGCTAAACGAAAATCCGAGGGTG10.7092198581560284No Hit
CTCCGAAGTTTCCCTCAGGATAGCCGGAGCTCGGACGCGAGTTCTATCGGG10.7092198581560284No Hit
AAATGAAGTCATTTGCATATCTCTAAAGGATGGTTTTGTTCAGAATGAGCT10.7092198581560284No Hit
AAATTCAGAGAAATAAAAAGGTCCTCTGAAGAAAAAAGTTAAACATTCGTG10.7092198581560284No Hit
TGCCACATCATCAATATGCACCATATTGTAAGTATCAAACAAATACCTGTC10.7092198581560284No Hit
CTTGTACTCTTCTGCCATACTTAATCTTGATTCGATTGAACACTTAGCGTG10.7092198581560284No Hit
TACTAAGGGTTTCTTATATGCTCAACACATGAGCGAAACCCTATAGCTGTC10.7092198581560284No Hit
AAAGAATACAATGTGAGGGATTCTTGGACTAAGGTTTTTACGGTTCAGAAA10.7092198581560284No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATACCCCCTC10.7092198581560284RNA PCR Primer, Index 27 (96% over 28bp)
GATCAACATAAGGAAACCTCAGAAGCTGTTTCTGGGTCATTGTCAGTTGAT10.7092198581560284No Hit
GTAAGAGATGGATCCATTTCGTTTTTGATCTTAATCAAAGCATCTAATACC10.7092198581560284No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT10.7092198581560284No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTAAGCCGTC10.7092198581560284No Hit
ATCTAAATCAGTCACTTGCAGTTTTCATTTCCCTTTCTTATATTTGTGTTT10.7092198581560284No Hit
CTTCCCGGTTCGCTCGCCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCC10.7092198581560284No Hit
CTTGCACTCAAAGCTTGTTAAGATAGTTAAGCGTGCATAAGTTAACTTCCA10.7092198581560284No Hit
GAGTTGATTACAGTTAGGGGAAAGTGTGAGCAATACATCTGTCTCTTATAC10.7092198581560284No Hit
GAGTTTCAAAAGTGATTTTTTTTTTCCCACAAAAGTTTCAACACTTAAGCT10.7092198581560284No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACCTGTCTCC10.7092198581560284Illumina PCR Primer Index 8 (96% over 27bp)
GAGTACGGGGTCATCTCCTTCATCTCTCAGGTAAAAAAACTGAGTCGAGAT10.7092198581560284No Hit
CTTATGTACTTGTGTTTTCAGTATTATTTATGTCATCATCATCCTCCTCTT10.7092198581560284No Hit
GTATACCTTTTTGTACCCATAAGTTAGCTCTCACTTATAAGCTGTCTCTTA10.7092198581560284No Hit
GGTATCAACGCAGAGTACGGCGGACACAGCAGCTTGGTCTCTTTCATTGGC10.7092198581560284No Hit
CTCCTTTACTATTCATGGCCACTATCATGGCTATTTCTTGCTTAATTTTTA10.7092198581560284No Hit
GTCTTTGTCTTCTTCCTTCTATCTTTTGTTCTTTCGTCTCAAACTCTTATC10.7092198581560284No Hit
TCTCGATTCCGTGTGACGGCTCAACAAAGCAGCCGACACGTCCTACCTATT10.7092198581560284No Hit
TGTCTGTGTTCTTCAAACCTGGAGATAAGTTTACAGAGATTGTAGGAAGTC10.7092198581560284No Hit
GGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATG10.7092198581560284No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGTATGCCGTC10.7092198581560284RNA PCR Primer, Index 27 (96% over 28bp)
ATCATATACATAACCTGCCTTAAAAGCATATTATTGTAGCTGTCTCTTATA10.7092198581560284No Hit
CTTTGTATGCGCCATTGTAGCACGTGTGTAGCCCCTGTCTCTTATACACAT10.7092198581560284No Hit
TCCAAAACAAGCTTAAGTCAGTAATAATCACTCTACTTTTTCTAAAACTCC10.7092198581560284No Hit
CAGTTCGGCTGGCGCGAGCCCCTGATGCTCTTCGTCCAGATCATCCTGATC10.7092198581560284No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCGTTATCTCGTATGCCGTC10.7092198581560284No Hit
ATACCATCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGC10.7092198581560284No Hit
CGTCTACATCGCCACCGTGGGCGCCAAGCTGTCTCTTATACACATCTCCGA10.7092198581560284No Hit
GTATAATGTACAAGAACAGAACATTTCTTAGGACTCCTAGTACTTTACTTT10.7092198581560284No Hit
GCTTTATCTTCATCAGTCATTGCCTTCCATCTAGCTCCAGCAGCCTTACCC10.7092198581560284No Hit
CTTATGTACTTGTGTTTTCAGTATTATTTATGTCATCATCATCCTGTCTCT10.7092198581560284No Hit
TATTTGCCTCTTTGAAGCGCTTTCCAACAGTATAGATCTCATGAATCCTGT10.7092198581560284No Hit
ACCTTTAACTTCATGTCCGAATAGACTAAAAGTGGTAAAAAGAAATTGGAA10.7092198581560284No Hit
ATCTTCATACTTGCATATTGATTCTTTCTCTTCCATTATACAATCACACCG10.7092198581560284No Hit
GATACTATGGTTTTACGCAAGATTACGGAAATATATTTGCGTTACCTACAT10.7092198581560284No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCCCGTACTCTGCGTT10.7092198581560284No Hit
GGAAGAAACAGAACATGAAACAGAGCAGAAACAGCAACACAAACAGAAACA10.7092198581560284No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGTCTCTTA10.7092198581560284No Hit
GGATGCAACAACAGTTTCTTCTTGTTGCTCTCTTTCTTGGGTTTTGTTTTA10.7092198581560284No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCGACAATCTCGTATGCCGTC10.7092198581560284RNA PCR Primer, Index 36 (95% over 21bp)
CATATATATGGCAGAAAAATTGGGGAAAATATGTGTTTTATGCTAAGAGAT10.7092198581560284No Hit
GACTACGCCTTTCGGCCTCGCCTTAGGGGTCGACTCACCCTGCCCCGATTA10.7092198581560284No Hit
TGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATAC10.7092198581560284No Hit
TATATGGGCAACGACATTGTGACAATACTGAAATTTCCTTCTGAATATCTT10.7092198581560284No Hit
AGTGTGCGTGAACCTAATTTAGGTTTCAGAAACATTTCCTCTAGAGTGTTT10.7092198581560284No Hit
AGATAATGCAATGTATGGTATATATAGCTTCAGTATCGCAAACAAATTTGT10.7092198581560284No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTCTACCCGCAA10.7092198581560284No Hit
CTTCTGTGTGTGTTTTTATGGTTTGGTCATTAAGATATCTCTGCAAAGTTT10.7092198581560284No Hit
GATTTACATGCCATATTGGATGATGCAGAATCTGCTGCTGCAAGAAGGAGA10.7092198581560284No Hit
CTAGAAACAGAGCGTATCTCTTTTGCTCCACCATTTTTTTTTGTTCTGGCT10.7092198581560284No Hit
CCTTATAGGTTTAATGTCTGGGTTCTCATGAGGATCTCTTTTATACATCTC10.7092198581560284No Hit
GTACTGGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGCTGTCTC10.7092198581560284No Hit
AAGGAGAAGAAAGACCATCATCACAACAAGCATCGAAAGACTGTCTCTTAT10.7092198581560284No Hit
TAATTAGCTTCATCTGTTTTGGATTTCAAACCATTAGCATTTGGCAAAGGA10.7092198581560284No Hit
GATATCCCAGGAAAATTAAGGCTTCTCTTAAACACTAAAAGGCATGTAATT10.7092198581560284No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATTCCGACCACC10.7092198581560284RNA PCR Primer, Index 27 (96% over 28bp)
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAACCCGCCCCTAAAACCCA10.7092198581560284No Hit
CCCAGTGTGTTTCGACACACTATCATTAACTTAATCCATATGTTAATCACC10.7092198581560284No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC10.7092198581560284No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCCCTTATCTCGTATGCCGTC10.7092198581560284No Hit
GTCTTGGTAGATTTTGATTTACGTACACCAACACTCTTTGACAACAAGTAT10.7092198581560284No Hit
CTACAGAACTGGTTGATCTCATGATGAATCTTGTCATAAATCCATGTGTTA10.7092198581560284No Hit
CTGAAAAGAGTCATTGATAACAAGGACAATGCAGAGAAGTTTAGGCTGTCT10.7092198581560284No Hit
GATCAGGACGACATGATCGACCGCGCAGGTAGCATCGCATTCCGTGACGGC10.7092198581560284No Hit
ACAAAACACTGGCTTTCAGGTTCTATCACCGAGGCACAGCCTGTCTCTTAT10.7092198581560284No Hit
CAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC10.7092198581560284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC10.7092198581560284No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers