FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_50.35100000044970.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_50.35100000044970.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155048
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC2305914.872168618750322No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT80225.173881636654455No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCG11830.7629895258242609No Hit
TTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTCT3460.22315669986068828No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2550.16446519787420671No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC1970.1270574273773283No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA1970.1270574273773283No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1610.10383881120685208No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC23950.043.58456845
GCCGTCT700.041.77972445
ATAGACT250.002096325636.0180786
ATGCCGT32950.031.61155144
ATCTCGT33950.030.4816437
TCTCGTA33950.030.4816438
GATCTCG33950.030.4816436
CGTATGC34050.030.45818941
CTCGTAT34000.030.43681139
TATGCCG34250.030.411743
CTGATCT34150.030.36899834
TGATCTC34300.030.36736535
CTACCGC34200.030.33438528
GTATGCC34200.030.324642
GCTGATC34150.030.30312233
ACCGCTG34300.030.30177730
ACTACCG34250.030.29010227
TACCGCT34250.030.29010229
CCGCTGA34200.030.2588231
TCGTATG34300.030.23618940
Produced by FastQC (version 0.11.2)