FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_5.351000000443e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_5.351000000443e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55009
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC1295723.554327473686122No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG6231.1325419476812886No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT5751.0452834990637896RNA PCR Primer, Index 44 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT820.14906651638822738RNA PCR Primer, Index 44 (95% over 23bp)
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG750.1363413259648421No Hit
GCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT670.12179825119525896No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC15350.042.94267745
ATGCCGT15900.041.45723744
GCCCACG16300.040.99196218
ACGAGAC16300.040.99196222
GACCCTA16450.040.891726
CCCACGA16350.040.86660419
CCACGAG16300.040.85394320
CACGAGA16300.040.85394321
ACCCTAG16500.040.76778427
CGAGACC16400.040.74201223
GAATATC16350.040.72900833
AGCCCAC16500.040.6314417
CCCTAGA16500.040.6314428
GAGACCC16450.040.61817624
AGAATAT16400.040.60483632
CTAGAAT16400.040.60483630
TAGAATA16400.040.60483631
ATATCTC16250.040.56431635
AGACCCT16600.040.52219825
GAGCCCA16700.040.4142616