Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0113_pb_5.351000000443e0.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 55009 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC | 12957 | 23.554327473686122 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG | 623 | 1.1325419476812886 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT | 575 | 1.0452834990637896 | RNA PCR Primer, Index 44 (95% over 21bp) |
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT | 82 | 0.14906651638822738 | RNA PCR Primer, Index 44 (95% over 23bp) |
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG | 75 | 0.1363413259648421 | No Hit |
GCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT | 67 | 0.12179825119525896 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 1535 | 0.0 | 42.942677 | 45 |
ATGCCGT | 1590 | 0.0 | 41.457237 | 44 |
GCCCACG | 1630 | 0.0 | 40.991962 | 18 |
ACGAGAC | 1630 | 0.0 | 40.991962 | 22 |
GACCCTA | 1645 | 0.0 | 40.8917 | 26 |
CCCACGA | 1635 | 0.0 | 40.866604 | 19 |
CCACGAG | 1630 | 0.0 | 40.853943 | 20 |
CACGAGA | 1630 | 0.0 | 40.853943 | 21 |
ACCCTAG | 1650 | 0.0 | 40.767784 | 27 |
CGAGACC | 1640 | 0.0 | 40.742012 | 23 |
GAATATC | 1635 | 0.0 | 40.729008 | 33 |
AGCCCAC | 1650 | 0.0 | 40.63144 | 17 |
CCCTAGA | 1650 | 0.0 | 40.63144 | 28 |
GAGACCC | 1645 | 0.0 | 40.618176 | 24 |
AGAATAT | 1640 | 0.0 | 40.604836 | 32 |
CTAGAAT | 1640 | 0.0 | 40.604836 | 30 |
TAGAATA | 1640 | 0.0 | 40.604836 | 31 |
ATATCTC | 1625 | 0.0 | 40.564316 | 35 |
AGACCCT | 1660 | 0.0 | 40.522198 | 25 |
GAGCCCA | 1670 | 0.0 | 40.41426 | 16 |