FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_49.35100000044957.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_49.35100000044957.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79684
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC37254.674715124742733No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT7860.9863962652477285No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1760.22087244616234128No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.18949851915064506No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.17569399126549873No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCG1380.17318407710456302No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1260.15812459213894886No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.1518498067366096No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1140.14306510717333468No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC1100.13804527885146328No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.13051553636865618No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1030.12926057928818835No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA930.11671100848350986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.11043622308117064No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA850.10667135183976709No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.10416143767883138No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA810.10165152351789568No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4300.041.3366345
GCCGTCT356.175973E-638.57089245
ATGCCGT5150.034.9509744
CGCTGAT5150.034.5140832
ATCTCGT5100.034.41128537
TCTCGTA5100.034.41128538
ACCGCTG5200.034.18221730
GCTGATC5200.034.18221733
CTACCGC5200.034.18221728
CTGATCT5200.034.18221734
CTCGTAT5150.034.07719439
ACTACCG5250.033.8566727
GATCTCG5250.033.8566736
TACCGCT5250.033.8566729
CGTATGC5200.033.74952741
GTATGCC5200.033.74952742
TCGTATG5200.033.74952740
CCGCTGA5300.033.5372731
GAGACTA5300.033.5372724
ACGAGAC5300.033.5372722