FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_48.3510000004493d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_48.3510000004493d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144000
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC3153521.899305555555557RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT87116.049305555555556No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG13880.9638888888888889No Hit
TTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCT6170.4284722222222222No Hit
ATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCTTC2160.15RNA PCR Primer, Index 20 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC207.0143264E-444.9979178
TGCCGTC35800.042.9239945
CATTAAT250.002097903536.0108341
TATACTT250.002097903536.0108345
ATGCCGT45000.034.14842244
CTCGTAT46250.033.2254939
ATCTCGT46250.033.1768437
CATCTCG46250.033.1768436
TCTCGTA46250.033.1768438
TATGCCG46450.033.13086743
CGTATGC46350.033.10526741
TCGTATG46300.033.0924240
GTATGCC46450.033.0824342
CCACGAG46600.033.07250220
GTGGACA46550.033.05969631
GGACATC46550.033.05969633
CACGAGA46650.033.03705621
CCCACGA46650.033.03705619
GACATCT46650.033.03705634
TGTGGAC46600.033.0242230
Produced by FastQC (version 0.11.2)