FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_47.35100000044913.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_47.35100000044913.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72519
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC3476447.93778182269474RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT799611.026075924930018No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG16082.217349935878873No Hit
TTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCT2060.28406348681035315No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGTTGACATCTCGTATGCCGTC1190.1640949268467574No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGACAT206.989592E-445.00000432
AGACCTT206.989592E-445.00000425
GACCTTT206.989592E-445.00000426
TGCCGTC35200.044.23295245
TGTTGAC250.002094141736.030
CCTGTTG250.002094141736.028
TGACATC250.002094141736.033
ATGCCGT43950.035.4266244
ATCTCGT45450.034.10891337
TATGCCG45800.034.09388743
GTATGCC45750.034.0819742
GCCCACG45950.034.0805218
CCACGAG45950.034.0805220
GAGCCCA45950.034.0805216
CACGAGA45950.034.0805221
ACGAGAC45950.034.0805222
CCGAGCC45950.034.0805214
TCCGAGC45950.034.0805213
CGAGACC45900.034.06862623
CGTATGC45800.034.0447641
Produced by FastQC (version 0.11.2)