FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_46.351000000448f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_46.351000000448f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2554
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC107342.012529365700864RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT1425.559906029757244No Hit
TTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCT250.9788566953797965No Hit
ATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCTTC210.822239624119029RNA PCR Primer, Index 20 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG180.7047768206734534No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGTTGACATCTCGTATGCCGTC90.3523884103367267No Hit
TATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCTT60.23492560689115116RNA PCR Primer, Index 20 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC50.19577133907595928No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG40.15661707126076743No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG40.15661707126076743No Hit
CTTATACACATCTCCGAGCACACGAGACCTGTGGACATCTCGTATGCCGTC30.11746280344557558No Hit
CTTATACACATCTCCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT30.11746280344557558No Hit
CTTATACACATCTCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCT30.11746280344557558No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1450.041.89655345
TATACAC1600.039.3753
TTATACA1600.039.3752
CTTATAC1600.039.3751
ATACACA1700.037.0588234
TACACAT1650.036.818185
GTATGCC1700.035.73529442
ATCTCGT1700.035.73529437
CACATCT1700.035.7352947
TATGCCG1700.035.73529443
CATCTCG1700.035.73529436
CATCTCC1700.035.7352949
ACACATC1700.035.7352946
TCTCGTA1700.035.73529438
ATGCCGT1700.035.73529444
CGTATGC1700.035.73529441
TCGTATG1700.035.73529440
CTCGTAT1700.035.73529439
ATCTCCG1650.035.45454410
CCTGTGG1650.035.45454428