Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0113_pb_46.351000000448f8.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2554 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC | 1073 | 42.012529365700864 | RNA PCR Primer, Index 20 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT | 142 | 5.559906029757244 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCT | 25 | 0.9788566953797965 | No Hit |
ATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCTTC | 21 | 0.822239624119029 | RNA PCR Primer, Index 20 (95% over 24bp) |
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG | 18 | 0.7047768206734534 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACCTGTTGACATCTCGTATGCCGTC | 9 | 0.3523884103367267 | No Hit |
TATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCTT | 6 | 0.23492560689115116 | RNA PCR Primer, Index 20 (95% over 23bp) |
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC | 5 | 0.19577133907595928 | No Hit |
TCTTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG | 4 | 0.15661707126076743 | No Hit |
TCTTAATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG | 4 | 0.15661707126076743 | No Hit |
CTTATACACATCTCCGAGCACACGAGACCTGTGGACATCTCGTATGCCGTC | 3 | 0.11746280344557558 | No Hit |
CTTATACACATCTCCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT | 3 | 0.11746280344557558 | No Hit |
CTTATACACATCTCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCT | 3 | 0.11746280344557558 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 145 | 0.0 | 41.896553 | 45 |
TATACAC | 160 | 0.0 | 39.375 | 3 |
TTATACA | 160 | 0.0 | 39.375 | 2 |
CTTATAC | 160 | 0.0 | 39.375 | 1 |
ATACACA | 170 | 0.0 | 37.058823 | 4 |
TACACAT | 165 | 0.0 | 36.81818 | 5 |
GTATGCC | 170 | 0.0 | 35.735294 | 42 |
ATCTCGT | 170 | 0.0 | 35.735294 | 37 |
CACATCT | 170 | 0.0 | 35.735294 | 7 |
TATGCCG | 170 | 0.0 | 35.735294 | 43 |
CATCTCG | 170 | 0.0 | 35.735294 | 36 |
CATCTCC | 170 | 0.0 | 35.735294 | 9 |
ACACATC | 170 | 0.0 | 35.735294 | 6 |
TCTCGTA | 170 | 0.0 | 35.735294 | 38 |
ATGCCGT | 170 | 0.0 | 35.735294 | 44 |
CGTATGC | 170 | 0.0 | 35.735294 | 41 |
TCGTATG | 170 | 0.0 | 35.735294 | 40 |
CTCGTAT | 170 | 0.0 | 35.735294 | 39 |
ATCTCCG | 165 | 0.0 | 35.454544 | 10 |
CCTGTGG | 165 | 0.0 | 35.454544 | 28 |