FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_44.351000000448b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_44.351000000448b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355505
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC189505.330445422708541RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT41511.1676347730692958No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC11540.3246086552931745No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC10860.3054809355705264No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA10050.28269644590090154No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG8490.2388152065371795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA8130.22868876668401286No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA6300.17721269743041587No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC5310.14936498783420768No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA5130.14430176790762436No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG4810.13530048803814293No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC4250.1195482482665504No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3920.11026567840114766No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC19600.043.9617545
TCGCCTC301.1381687E-437.5009428
CCCCCGG301.1381687E-437.5009420
ACACGCG250.002106351335.9958347
CGGGATT250.002106351335.99583416
CTCCTAG250.002106351335.99583412
ATGCCGT24100.035.75312844
TATGCCG25300.034.05732743
CGTATGC25100.033.97017341
GTATGCC25300.033.87948242
GCCGTCT401.5587815E-533.74609845
TCTCGTA25350.033.63516238
CTCGTAT25450.033.50300239
ATCTCGT25500.033.4373137
CATCTCG25550.033.37187636
TCGTATG25550.033.37187640
GGACATC25700.033.35686533
GTGGACA25950.033.2956331
TGTGGAC25950.033.20892330
ACCTGTG25900.033.18615727
Produced by FastQC (version 0.11.2)