FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_43.351000000448a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_43.351000000448a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC43.418803418803419RNA PCR Primer, Index 20 (95% over 21bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA21.7094017094017095No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT21.7094017094017095No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA21.7094017094017095No Hit
GCAGAGTACTTTTTTCTTTTTTTTTTTTTTTTTTTTTTCTGTTAAACACAC10.8547008547008548No Hit
GTACGGGAAGAGAAGATAAGAGAAGAGCAGAAAAGGTAGAAGATGACAGAG10.8547008547008548No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTCTGTCTCTTATACACATCT10.8547008547008548No Hit
CTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGCTGTCTCTTATACA10.8547008547008548No Hit
GTATTGAGTATCTTAAGTACTGTAGAAAAGATGTCACTTCTTCCTTTCTGT10.8547008547008548No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGTGGTCATCTCGTATGCCGTC10.8547008547008548RNA PCR Primer, Index 20 (95% over 21bp)
CTCCAGAGCTCACGCTTGTTACGCAGACCATACTCACCAACCAGCCTGTCT10.8547008547008548No Hit
CACCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC10.8547008547008548No Hit
AAAAAGTACTCTGCGTTGCCCGTACTCTGCGTTGATACCTGTCTCTTATAC10.8547008547008548No Hit
GACATATACATACACACACATTGCTATGTACCTGTCTCTTATACACATCTC10.8547008547008548No Hit
ACCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACGA10.8547008547008548No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC10.8547008547008548No Hit
ATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACAC10.8547008547008548No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTGGACATCTCGTATGCCGTC10.8547008547008548No Hit
CCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGGAAACCGGGTT10.8547008547008548No Hit
ACTTGGTGCAGGTAAATATACAATCTGTACAAACACAAGCCACCTGCAAAG10.8547008547008548No Hit
ACATTGTTTCACACATACATCAAACAGGCCAAAAAAAATAAACAGCAACCT10.8547008547008548No Hit
GGTATCAACGAAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG10.8547008547008548No Hit
GACCAGGACGCATCGCCGGCCCCCATCCGCTTCCCTCCCGACAATTTCAAG10.8547008547008548No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAGTACCTGTCTC10.8547008547008548No Hit
GCAACAGGGACATAAGTAACCAAGGTGTAAAGCTTGTTAGCTGTCTCTTAT10.8547008547008548No Hit
ATGAAGCTCAGTCCAAGAGAGGTATCCTCACTCTGAAGTATCCAATCGAAC10.8547008547008548No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTATCCCCTT10.8547008547008548No Hit
CAGTTTCAGATGATCTCAGTACACTAGTAGTAGTAGTAGTAGTAGCTGTCT10.8547008547008548No Hit
ACATAATCATAAGCACTTTCAGTCTAAACACCTGTCTCTTATACACATCTC10.8547008547008548No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGACACCTA10.8547008547008548RNA PCR Primer, Index 27 (96% over 28bp)
GTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACCCCCC10.8547008547008548No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAAAAATA10.8547008547008548No Hit
ATCTCAATGTGATGTTGAAGTTGAATCCTTATGCTAAGACCTGTCTCTTAT10.8547008547008548No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATTACCATCTCGTATGCCGTC10.8547008547008548No Hit
ATGTGGAACACAAGGTTGAAACTTTTTCTGGTGTCTATAAGCTGTCTCTTA10.8547008547008548No Hit
TCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATAC10.8547008547008548No Hit
TCTTTGTGGAGCTAGTTTCAAAGTTGATGCTTGTTCTAAGACTGTCTCTTA10.8547008547008548No Hit
TCTTTCCTCACCACATTTGGAAAAACATCTGTTAAAGCCTTTAACCGCCTT10.8547008547008548No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAC10.8547008547008548No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAAACATAAAA10.8547008547008548Illumina PCR Primer Index 8 (96% over 27bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATTTTATTTCTC10.8547008547008548Illumina PCR Primer Index 8 (96% over 27bp)
GATTAAGACTCTGAAACCAGATATTCATAGGCCCTGTCTCTTATACACATC10.8547008547008548No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTGATACCCCCC10.8547008547008548No Hit
TGTCTTATCTCGTAAGCCGTCTTCTGCTTGAAAAAAAAAACATAAGCCTAC10.8547008547008548Illumina PCR Primer Index 10 (96% over 27bp)
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG10.8547008547008548No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTGCTTTGATACCCC10.8547008547008548No Hit
ATTGTGGAGGAAGAGAAATCTCAGATTGAATCCATCAAAGAGCAATTGAAA10.8547008547008548No Hit
GGGGAAACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCACAGCTT10.8547008547008548No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTC10.8547008547008548No Hit
AAAGTACTCTGCGTTGATACCCGTACTCCTGTCTATTATACACATCTCCGA10.8547008547008548No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTAC10.8547008547008548No Hit
GAAGAGAGTGGAGTGTATTGGAGTGGAGTGCAATGGAGCTGTCTCTTATAC10.8547008547008548No Hit
TGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACCTGTCTCTTATACAC10.8547008547008548No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC10.8547008547008548No Hit
CAATTGGTGAATCCAGAAGGAAGGGTATATGGGGGTTCATTATACCTGTCT10.8547008547008548No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCC10.8547008547008548No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCCGGCAACCA10.8547008547008548Illumina PCR Primer Index 8 (96% over 27bp)
GCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGCTGTCTCTTATACACA10.8547008547008548No Hit
ATTTCGAATACTGTAAAGGCAGCATCTCATCAGTGAAAGGAAATGTATCTG10.8547008547008548No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG10.8547008547008548No Hit
GATTTGCTCTTAGAGAATGGAGAAGACAAAATGCAATTCAACTTGAGGAGA10.8547008547008548No Hit
GTATCAACGCAGAGTACGGGGTCTGTCTCTTATACACATCTCCGAGCCCAC10.8547008547008548No Hit
GAGAAAACTTACAATCCTTAAAGTGCACAATGAAAGCTGTCTCTTATACAC10.8547008547008548No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTGATACCCCCC10.8547008547008548No Hit
CTGTTTTGGTGTCAAAAGTTTGTGTCTAGTTATCTTAAGCACATGCTTGTA10.8547008547008548No Hit
GTTAATACCTTCAGAATGCTTCAAATAGTGCCCTGTCTCTTATACACATCT10.8547008547008548No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACCAAAGCA10.8547008547008548RNA PCR Primer, Index 27 (96% over 28bp)
AGATATAACGACACAACATTCGCTAAAAGTAATGGAAGAGATGACACAATC10.8547008547008548No Hit
GTAACAACCATGGGAGATTTTCCAAATACCTCCATCTTATTCCTGTGATCT10.8547008547008548No Hit
CCTCAAGGAACTAGAAAAACAAGAACAAAACAAACCCTGTCTCTTATACAC10.8547008547008548No Hit
TTCTTATACAAAAGGGAGAGAAAGGAGAGCGTTTAAGCTGTCTCTTATACC10.8547008547008548No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTCAATGC10.8547008547008548No Hit
GTACGGGATCTATCTATCTATATACCCAAAGAGTCGCTTTGTTCCTGATTC10.8547008547008548No Hit
CTCCAAGTTTGTGTTTAGAGTTAGATCATTTGTGTCTAAAATCTATAAAAT10.8547008547008548No Hit
ATATAAATCTCGTATGCCGTCTTCTTCTTGAAAAAAAATCGCTAAGGAGTG10.8547008547008548Illumina PCR Primer Index 4 (96% over 25bp)
GCTATTACGCTTTCTTTAAATGATGGCTGCTTCTAAGCCAACCTGTCTCTT10.8547008547008548No Hit
AAGCAGTGGTATCAACTCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAA10.8547008547008548No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGCAGTCTCTTA10.8547008547008548RNA PCR Primer, Index 36 (96% over 29bp)
ATGTATAACAGTCAATTAATCCCGTACTCTGCGTTGATACCCTGTCTCTTA10.8547008547008548No Hit
AGATTATTTAGAAGGCAACCCTGATTTTAGCTTAACAGATGTTGGTACAAT10.8547008547008548No Hit
AAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTATAC10.8547008547008548No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTCTGTCTCTTATACACAT10.8547008547008548No Hit
GTAGGGCTTGTTATCAGTATGGTTTACAGGGACACTTTAAAAAAGACCTGT10.8547008547008548No Hit
GCATATCAATGATCTGGTACTCCTTAACCGGGAGTGAATGAAAGATAGAAC10.8547008547008548No Hit
AAATAACATGGTAGATGCTGGGACAAATGGTAGCTTGTCACTAATCCTGTC10.8547008547008548No Hit
GTGCTAATCTGCGATAAGCGTCGGTACGGTGATATGAACCGTTATAACCGG10.8547008547008548No Hit
AAGCTTTTATCTACAGATCATATCCCAGAGCTATCAATTGTCCTGTGACTG10.8547008547008548No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAGCACATCTCGTATGCCGTC10.8547008547008548No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGA10.8547008547008548No Hit
GGTATCAACGCAGAGTACGGGGTAACGGGGCTAAACCATGCACCGAAGCTG10.8547008547008548No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCTATTGAGTG10.8547008547008548Illumina PCR Primer Index 8 (96% over 27bp)
GCTGCGTCCCGATGCCGGTCGGGTCGAGGTGCTCGGCCACTGTCTCTTATA10.8547008547008548No Hit
GATATTTCACTTAACATAATTTCAAAGTCAGTCTGTGGTCTAAGATGAGCA10.8547008547008548No Hit
GGCTTGGTTGGAGTCATCTTCACCATACCAGCATCACCATTCTTCAAGAAT10.8547008547008548No Hit
ATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGT10.8547008547008548No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA10.8547008547008548No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTAATAAACC10.8547008547008548RNA PCR Primer, Index 27 (96% over 28bp)
TACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCCTG10.8547008547008548No Hit
GAATTAGCTTTCTCACCCAGAACCTGATTATCAATCGACTACAACAATGCA10.8547008547008548No Hit
GTATAAGGATGCATTTATGAAAGCAAATCCTGGCTACAAATGGTGCTGTCT10.8547008547008548No Hit
CCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCC10.8547008547008548No Hit
GTACGGGAAGCAGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8547008547008548No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAACAAAA10.8547008547008548Illumina PCR Primer Index 8 (96% over 27bp)
CCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCT10.8547008547008548No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTAAACTGTCTC10.8547008547008548No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC10.8547008547008548No Hit
CTTATACACATCTCCGCGCCCACGAGACCTGTGGACATCTCGTATGCCGTC10.8547008547008548RNA PCR Primer, Index 20 (95% over 21bp)
TATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACG10.8547008547008548No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC10.8547008547008548No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGTTGATATCTCGTATGCCGT10.8547008547008548No Hit
GTATTTAGTTTCATAGCTTATAGTTTTGTGTTTGCGGAGAAAAAAAACAAA10.8547008547008548No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers