H23LNBCXX_l01n01_bir_0113_pb_41.35100000044864.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0113_pb_41.35100000044864.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25339
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC	1357	5.3553810331899445	RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT	248	0.9787284423221122	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG	65	0.256521567544102	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	54	0.21311022534433086	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44	0.17364536879908443	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	41	0.1618059118355105	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39	0.15391294052646118	No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC	37	0.1460199692174119	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32	0.12628754094478867	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31	0.12234105529026403	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	29	0.11444808398121474	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	28	0.11050159832669008	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	180	41.25	45
CATCTCG	220	33.75	36
TGGACAT	220	33.75	32
CCTGTGG	230	33.260868	28
GGACATC	230	33.260868	33
CACGAGA	230	33.260868	21
ACCTGTG	230	33.260868	27
CTGTGGA	230	33.260868	29
ATCTCGT	225	33.0	37
TATGCCG	225	33.0	43
TCTCGTA	225	33.0	38
ATGCCGT	225	33.0	44
CGTATGC	225	33.0	41
CTCGTAT	225	33.0	39
CCACGAG	235	32.553192	20
GACATCT	235	32.553192	34
ACGAGAC	235	32.553192	22
TGTGGAC	235	32.553192	30
GTATGCC	230	32.282608	42
TCGTATG	230	32.282608	40