FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_40.3510000004484b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_40.3510000004484b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT36.382978723404255No Hit
CATGTTTGGTGCTCTCTTAACTCCCATTACCTGTCTCTTATACACATCTCC12.127659574468085No Hit
ATATCAAGTTGTCATTAGGACCATGATCTCTCTGAAGGCTAATCTGTCTCT12.127659574468085No Hit
GCCGTATTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCC12.127659574468085No Hit
CCTTAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGA12.127659574468085No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTAAACCCCC12.127659574468085Illumina PCR Primer Index 8 (96% over 27bp)
ACGGGGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGCTGTCTC12.127659574468085No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT12.127659574468085No Hit
GTACGGGATGGTTATTTCAAAGACAGTGTTTCCATATTCTGTTTCTAATAA12.127659574468085No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACCTGTCTCGTATACA12.127659574468085No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTGAAACCCCTTCCCC12.127659574468085No Hit
CAGTAAAATTGGAGAGGATTCTTTTGCATTGAATAAACTCTGTCTCTTATA12.127659574468085No Hit
GGACAATAACTACCGAATCTTTCATCTAAGATGCAGCAGTTGTCTCTGGTA12.127659574468085No Hit
GTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAAGGACTGTCC12.127659574468085No Hit
GTATCAACGCAGAGTACGGGCTGTCTCTTATACACATCTCCTAGCCCACGA12.127659574468085No Hit
CTTATACACATCTTCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC12.127659574468085No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC12.127659574468085No Hit
ATGGTGGGTTGCGCTTATCTTGGGCACTTCTGGGTTATATGTACTGCTACA12.127659574468085No Hit
GTTGAGAACTGTGCGTTATCTAGAGATGTATCTTCTTCACGTAAACGTTCA12.127659574468085No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCCTGTCTCTTATA12.127659574468085No Hit
TGATGATACTGCTCACATATGGATTACATATGCCGGTAGCACTGTCTCTTA12.127659574468085No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCCCC12.127659574468085No Hit
GGTATCAACGCAGAGTACGGGTAGGTAGCGCCTCGTGAATTCAACTCCGGG12.127659574468085No Hit
CCGCAAGGCTGAAACTCAAAGGAATTGACGGGGCCCCGTACTCTGCGTTGA12.127659574468085No Hit
CTTTAAAGAAAGCGTAATAGCTCACTGATCGAGTCGTCCCTGTCTCTTATA12.127659574468085No Hit
CAGTGGTTGTGGAGGAAGAGAAGAAGCCAGAGGCCTGTCTCTTATACAACC12.127659574468085No Hit
GATTAACTTTTTAGGGAACAAGTCATTTAAATTCTTGAACCAGTTTCCATC12.127659574468085No Hit
GTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTC12.127659574468085No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTGGAAAAAAAAAACAAAGACCGAA12.127659574468085Illumina PCR Primer Index 4 (96% over 25bp)
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATCTGTCTCTTAT12.127659574468085No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCAA12.127659574468085No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC12.127659574468085No Hit
CTTATACACATCTCCGAGCTTATACATCTCGTATGCCGTCTTCTGCTTGAA12.127659574468085RNA PCR Primer, Index 39 (96% over 31bp)
CTTATACACATCTCCGAGCCCACGAGACAGGAATTAATCTCGTATGCCGTC12.127659574468085No Hit
CTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTTATTCCGATTAACGCT12.127659574468085No Hit
ATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAACTGTCTCTTATAC12.127659574468085No Hit
GATGTCATCTTACTGCTTAATCATTCATGTATCTGACTGTCTCTTATACAC12.127659574468085No Hit
GTTCTTCTTAGAGGACCTAAGAATTCACGTGAAGCAGTGAAGCATTTCGGA12.127659574468085No Hit
CGATTGTACCCTCACGCATGGTGCCCACAGGGAGCACATTGCCAATGTTGA12.127659574468085No Hit
TTTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATAC12.127659574468085No Hit
GATACCAATGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC12.127659574468085No Hit
CTCCACCGCTTGTGCGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCG12.127659574468085No Hit
TAGCCTTCCTAATGATTAGTGCAGTATCGAATGGACAAGCTGTCTCTTATA12.127659574468085No Hit
GATGATTTCTTCAGCATGTTAGAGCAGTGAGGAATGGTTTTAGTCTCATAA12.127659574468085No Hit
GACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACAC12.127659574468085No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers