FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_39.35100000044821.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_39.35100000044821.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95355
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC2665427.95238844318599No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT56775.953542027161659No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG53145.572859315190604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC29250.043.7687245
CCGTCTT356.1887276E-638.5709817
GTCTTCT301.1321671E-437.4995619
ATGCCGT34700.036.89438244
CATACAA250.00209757335.999582
AACTCAC250.00209757335.9995821
TGAAAAA401.545789E-533.74960729
CGTCTTC352.8013397E-432.14248318
CTTGAAA352.8013397E-432.14248327
CGTATGC40050.032.07827841
TATGCCG40100.032.0382843
TCGTATG40050.031.9659240
TCTCGTA39700.031.96436338
GTATGCC40200.031.95858442
CTCGTAT39900.031.91691839
GATCTCG39700.031.7943436
ACAGGAT40550.031.7937127
CACGAGA40650.031.77084721
ACGAGAC40650.031.77084722
CCACGAG40650.031.77084720
Produced by FastQC (version 0.11.2)