FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_38.35100000044807.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_38.35100000044807.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45683
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC531011.623579887485498No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT23725.19230348269597No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG6041.3221548497252809No Hit
TTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTCT480.10507190858743953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5600.045.00000445
ATGCCGT8350.030.17964244
TATGCCG9050.027.84530443
GTATGCC9150.027.54098342
CGTATGC9150.027.54098341
ATCTCGT9000.027.537
GATCTCG8950.027.40223536
CTCGTAT9200.027.39130239
TCTCGTA9050.027.34806838
TCGTATG9250.027.24324240
ACGAGAC9350.026.9518722
GCCCACG9400.026.8085118
CCACGAG9400.026.8085120
GAGCCCA9400.026.8085116
CACGAGA9400.026.8085121
CCCACGA9400.026.8085119
CGAGACA9350.026.7112323
ACAGGAT9350.026.7112327
AGCCCAC9450.026.66666817
CGAGCCC9450.026.66666815
Produced by FastQC (version 0.11.2)