FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_37.351000000447df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_37.351000000447df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13889
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC1030.741594067247462No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA230.16559867521059832No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC200.14399884800921592No Hit
ATCCTACACAGATCGGTTCAAAGTCCAATGCAAAGCTACAGTAAAGGTTCA200.14399884800921592No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC190.13679890560875513No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.13679890560875513No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA180.12959896320829434No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.12239902080783352No Hit
GTGTAGGATAGGTGGGAGGCTTTGAAGCGTGGACGCTAGTTCACGTGGAGC160.11519907840737274No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATG160.11519907840737274No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC140.10079919360645115No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC206.794404E-445.042
ATCTCGT206.794404E-445.037
TGCCGTC206.794404E-445.045
CCACGAG206.794404E-445.020
TGATCTC206.794404E-445.035
TATGCCG206.794404E-445.043
GATGTGA206.794404E-445.031
CCCACGA206.794404E-445.019
ATGTGAT206.794404E-445.032
TCTCGTA206.794404E-445.038
ATGCCGT206.794404E-445.044
CGTATGC206.794404E-445.041
TCGTATG206.794404E-445.040
GCCCACG250.002036199236.018
AGGATGT250.002036199236.029
CACGAGA250.002036199236.021
GATCTCG250.002036199236.036
GTGATCT250.002036199236.034
CGAGACA250.002036199236.023
CTCGTAT250.002036199236.039
Produced by FastQC (version 0.11.2)