FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_35.351000000447a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_35.351000000447a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC55.88235294117647No Hit
ATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCCGGGCCTGTCTCTTAT11.1764705882352942No Hit
TTTTTTTTTGCGTTGATACCACCCCGTACTCTTCGTTGATACCTGTCTCTT11.1764705882352942No Hit
GAACTTGAGATGGAAAAACAGAAACTACATGAGAGGTGTAGCAAATATCAA11.1764705882352942No Hit
GAAGAAGCTTCATCTCAACCTCCGTCATGTTTTAGAAACCTTTTATCCTGT11.1764705882352942No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGAATGCCGTC11.1764705882352942RNA PCR Primer, Index 27 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC11.1764705882352942No Hit
AGATAGATGTTTGACTTGGGTTGATGTTTTGTTCTAGCCCTGTCTCTTATA11.1764705882352942No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAG11.1764705882352942No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACCTGTCTCTTATACACAT11.1764705882352942No Hit
GAACTCCTCCGTTACACTTTGGGAGGAGACCGCCCCAGTCAAACGGCTGTC11.1764705882352942No Hit
ATCTTCAAGTTAAAAATGAAGATAAAGGGACAAAGGCTGTCTCTTATACAC11.1764705882352942No Hit
AAGGTACTCTGCGTTGACCCGTACTCTGCGTTGATACTGTCTCTTATACAC11.1764705882352942No Hit
GTGCTGGAAGATTAATTGATGGGGTGCAAGCTCTTGATTGAAGCTGTCTCT11.1764705882352942No Hit
TATACTTACCGCCCGTTTTGGAGACGCTATTAAATAGGTAGGTTATACACC11.1764705882352942No Hit
GTCTTTATTTAACTAAGAAGAAAGTGTTGAATACAAAGACTGCTAACTGTC11.1764705882352942No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT11.1764705882352942No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACACCGC11.1764705882352942Illumina PCR Primer Index 8 (96% over 27bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAAACA11.1764705882352942RNA PCR Primer, Index 27 (96% over 28bp)
GAAGCAGCTCGAGCAGTCCGCTGACAGCCGACGGGTTCGGAACTGGGACCC11.1764705882352942No Hit
GTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCACTTATACACATC11.1764705882352942No Hit
TATCAACGCAGAGTACGGGTGGGATCAACGCAAAAAAACTGTCTCGTATAA11.1764705882352942No Hit
CCTTTCCATATCATGAGTGCAGAAGACACGATCATGGCGAAGTTTAAAACC11.1764705882352942No Hit
GATGGTATTGCACATGGGTTCGACATATGTAGCTGTCTCTTATACACATCC11.1764705882352942No Hit
CCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCT11.1764705882352942No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTCTGTCTC11.1764705882352942No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC11.1764705882352942No Hit
GTATCAACGCAGAGTACGGGGTATAAACGCAGAGTACTTTTTCTGTCTCTT11.1764705882352942No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA11.1764705882352942No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCTAAGCCGTC11.1764705882352942RNA PCR Primer, Index 27 (96% over 28bp)
GTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCC11.1764705882352942No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGCTGTCTCTT11.1764705882352942No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAACAACGAAAAATGAT11.1764705882352942Illumina PCR Primer Index 8 (96% over 27bp)
ATATTGGAACACTTCTGAAAAACACCAATTGGATGAGGATCTTCAAGAACT11.1764705882352942No Hit
GTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGCTGTCTC11.1764705882352942No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCTCTTATAC11.1764705882352942No Hit
CACTCCTTCAACTGTAAAACTGATTTCTTAGCCATAGACGCATATGCCTGT11.1764705882352942No Hit
CTTATACATTTGATAAAGTAAGGCATGGTTGTGGTTAATCTGGTTTATTTT11.1764705882352942No Hit
GCATATGGGACTCACCTACCAAACAACTTTCTGTCGTTGTGCCTTCATACA11.1764705882352942No Hit
CTCTGAGCGTTTCCTCTTCCTAAGGGCTTTCTCTCTCTCTCTCTTTTTTTT11.1764705882352942No Hit
TATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAGAGTACCTGTCTCTT11.1764705882352942No Hit
GTATCAACGCAGAGTACGGGCAAATTGGTGTTCTAAATGTCTTAAGAACCT11.1764705882352942No Hit
CTTTGATTACTCTGTGAACTATTGGAATGTCTCGCCCTTCAACCTGTCCCC11.1764705882352942No Hit
GGGTTAAACGCAAGCATAATGCTTTGTTTAAAGGATTACGTCTGTCTCTTA11.1764705882352942No Hit
TCCCCATAGAATCTGATGAGTTCTGTGGTAGAATTGTCCAAGCTCTGTCTC11.1764705882352942No Hit
GTGATGCTGAGAAGTTTCGTTGATAACCTGTCCATCTCTAGTTTCAACCGT11.1764705882352942No Hit
GTCCTCACAAGATTCAAGGGATAGGAGCTGGTTTTATACCAAGTGTATTGA11.1764705882352942No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA11.1764705882352942No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTCCGAACCC11.1764705882352942Illumina PCR Primer Index 8 (96% over 27bp)
TTGTAGATGTGGTTTGACTATTTCTGTATGTCTCCATCTATCTGTCTCTTA11.1764705882352942No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAGTACTCCTGTCT11.1764705882352942No Hit
GGACCACATTTACAAACTGATAGGAAGGAGCACACAGTTACATGTCCATTT11.1764705882352942No Hit
GATGCATTGTCATCAGATTCCAATATGATGATCATCAGCTGTCTCTTATAC11.1764705882352942No Hit
AAAAAAAAAAAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCC11.1764705882352942No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCTCTTAT11.1764705882352942No Hit
AACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCCTGTCTCTTATA11.1764705882352942No Hit
GAGTAAGGAGACGTGTGTGTGTGTGAAAACTCAGTCATAATAGCTGTCTCT11.1764705882352942No Hit
AAAGTACTCTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCT11.1764705882352942No Hit
TCACAGTATAACACCCATACAAGCTTATCGGGTACAATACCTGTCTCTTAT11.1764705882352942No Hit
CTTGAACTTTGAAGTTACTAAGGATGACGGCAAGAATTAATGTCATAAAAC11.1764705882352942No Hit
TTTTTTTTTGCGTTGATACCACTCCCGTACTCCGCGTTGATCTGTCTCTTA11.1764705882352942No Hit
GTATCAACGCAGAGTACGGGACGCAGAGTACTTTTTTTTTAAGCAGTGGTA11.1764705882352942No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATCTGTCTCTTATA11.1764705882352942No Hit
TTCTTATACATCTCCTCCATGTCTGGAGTTACGCTGTTCTTTATGTATTGA11.1764705882352942No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA11.1764705882352942No Hit
GTACAGTCACCAGAAGTACACAGTTATCAAAAATGCACACACTTCACTTGC11.1764705882352942No Hit
ACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCTGTCTCTTATA11.1764705882352942No Hit
GTGCTAATATCTTTTTGGTTGTTTGTAATTGTGTTCATATGGCTGTCTCTT11.1764705882352942No Hit
AATGGGTACAGAAGAATTCATCTTCGTGAATGAAACCTAGAGCTGTCTCTT11.1764705882352942No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTAAACACAT11.1764705882352942Illumina PCR Primer Index 8 (96% over 27bp)
CAACTACAGGGAATGAAAAGTTCAAAAAGTAGATCCTACAAGATGTAACGA11.1764705882352942No Hit
TTCAAATAGAATCCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT11.1764705882352942No Hit
TATCAACGCAGAGTACGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATA11.1764705882352942No Hit
GACGAACCCGTTCACCCATCTTTTTGATGCGTTCAGATCTTGTCAAAAACT11.1764705882352942No Hit
TGCTAACGCAATGTGATTTCTGCCCGTACTCTGCGTTGATACCTGTCTCTT11.1764705882352942No Hit
GGTTGGGGATACACACGGCAGAATTCGGAAACAGGGTATAGATCAACAAAA11.1764705882352942No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC11.1764705882352942No Hit
GTACTTTTTTTTTTTTTTTTTTTTGGGTTTAAACCCCTGCCCGTCCTCCTT11.1764705882352942No Hit
TATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACG11.1764705882352942No Hit
GGATCATAAGGAGTGGTTTGGGAAACCTTTGAAAGATTCATTTTACTACAA11.1764705882352942No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTGTTTAAACCCCCCCCTTCCCCTGG11.1764705882352942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACA50.045.09
ACTGTCC50.045.045
GTGTTTC50.045.03
CACACTA50.045.012
AATCCAT50.045.029
ATCCATA50.045.030
TCGACAC50.045.08
ATGACTG50.045.042
TGACTGT50.045.043
ACTGAAT50.045.025
AACTGAA50.045.024
ATAGGTT50.045.034
TTCGACA50.045.07
TGTTTCG50.045.04
ACACTAT50.045.013
GTTTCGA50.045.05
TAATGAC50.045.040
ACACACT50.045.011
GACACAC50.045.010
CTGAATC50.045.026
Produced by FastQC (version 0.11.2)