FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_34.3510000004478f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_34.3510000004478f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15783
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC3672.3252867008806946No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT1080.6842805550275612No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT790.500538554140531No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA640.4054995881644808No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT530.33580434644871066No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG350.22175758727745043No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA290.18374200088703035No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC290.18374200088703035No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA250.1583982766267503No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC240.1520623455616803No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT230.14572641449661028No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT220.13939048343154026No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT220.13939048343154026No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGACTGTCTCTTATAC210.13305455236647026No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC210.13305455236647026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC200.12671862130140024No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA200.12671862130140024No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT190.12038269023633022No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAA190.12038269023633022No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG180.11404675917126021No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT180.11404675917126021No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA170.1077108281061902No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA170.1077108281061902No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC170.1077108281061902No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT170.1077108281061902No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT160.1013748970411202No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACTGTCTCTTAT160.1013748970411202No Hit
GTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTA160.1013748970411202No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC453.3651304E-1045.00000445
ATGCCGT605.6206773E-933.7544
GTATCAA1150.033.2608681
GGATGTG651.22727215E-831.15384930
GCCCACG651.22727215E-831.15384918
CCACGAG651.22727215E-831.15384920
CACGAGA651.22727215E-831.15384921
CCCACGA651.22727215E-831.15384919
CAGGATG651.22727215E-831.15384928
GGTATCA300.0049963929.9999981
GTATGCC702.5251211E-828.9285742
ATCTCGT702.5251211E-828.9285737
AGGATGT702.5251211E-828.9285729
TGATCTC702.5251211E-828.9285735
TATGCCG702.5251211E-828.9285743
GATGTGA702.5251211E-828.9285731
GAGCCCA702.5251211E-828.9285716
ATGTGAT702.5251211E-828.9285732
ACGAGAC702.5251211E-828.9285722
GATCTCG702.5251211E-828.9285736
Produced by FastQC (version 0.11.2)