FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_33.35100000044765.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_33.35100000044765.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15435
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC12207.904114026563006No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT4452.8830579850988016No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG1591.0301263362487851No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA210.13605442176870747No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA170.1101392938127632No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC160.10366051182377713No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT160.10366051182377713No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1150.043.0434845
ATGCCGT1850.026.75675844
TATGCCG2000.024.7543
GTATGCC2050.024.14634142
ATCTCGT2050.024.14634137
TCTCGTA2050.024.14634138
CGTATGC2050.024.14634141
TCGTATG2050.024.14634140
CTCGTAT2050.024.14634139
CCACGAG2100.023.5714320
TGATCTC2100.023.5714335
CACGAGA2100.023.5714321
CCCACGA2100.023.5714319
ACGAGAC2100.023.5714322
CGAGACA2100.023.5714323
ACAGGAT2100.023.5714327
CAGGATG2100.023.5714328
GATGTGA2150.023.02325631
GATCTCG2150.023.02325636
GAGACAG2150.023.02325624
Produced by FastQC (version 0.11.2)