FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_32.3510000004473f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_32.3510000004473f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174393
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC2727115.637668943134187No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT44472.549987671523513No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG7120.4082732678490536No Hit
TTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCT5770.3308619038608201No Hit
ATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCTTC2000.11468350220479033Illumina PCR Primer Index 3 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC30350.042.843845
ATGCCGT35850.036.2080844
GCTAGAC250.002100772736.0050473
TCGGTCA250.002103733635.9947219
TCCGAGC36300.035.94514513
CGAGCCC36300.035.94514515
CCGAGCC36400.035.908214
GCCCACG36450.035.8589418
GAGCCCA36450.035.8589416
AGCCCAC36550.035.8223817
ACGAGAC36450.035.80748722
CATCTCG36200.035.79585636
CCCACGA36400.035.7845919
CTCGTAT36250.035.74648739
TCTCGTA36200.035.7337138
CACGAGA36500.035.69678521
CGAGACC36500.035.69678523
ATCTCGT36250.035.68442537
GACCATC36450.035.68401326
CTCCGAG36700.035.61467412
Produced by FastQC (version 0.11.2)