FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_31.35100000044715.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_31.35100000044715.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169082
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC9467955.99590731124543No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT1985711.744005866975787No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG39402.3302303024567963No Hit
TTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCT7310.4323346068771365No Hit
ATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCTTC2250.13307152742456324Illumina PCR Primer Index 3 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCCG253.8814724E-544.99053610
TGCCGTC99550.043.94147545
ACACTCC301.137215E-437.492119
ACTCCGA301.137215E-437.4921111
ATGCCGT119950.036.5245944
CGAGACT250.00210115636.0030823
CTCTCCG250.002104210235.9924289
AGCCCAC124150.035.3252117
CTCCGAG124850.035.31495712
GAGCCCA124350.035.3122316
TATGCCG124100.035.2850543
TCCGAGC124850.035.26090213
CCCACGA124250.035.2605619
CCGAGCC124600.035.25943414
CGAGCCC124500.035.2516215
GCCCACG124300.035.24637618
GTATGCC124350.035.232242
CGTATGC124300.035.2282741
ACGAGAC124800.035.2133522
TCTCGTA123650.035.2132838
Produced by FastQC (version 0.11.2)