FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_30.35100000044708.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_30.35100000044708.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189285
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC6331933.45167340254115No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT2248811.88049766225533No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG26231.38574107826822No Hit
TTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCT9240.48815278548220936No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG1980.10460416831761629No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGGA207.0183433E-445.012
TGCCGTC67250.043.82899545
TACGGGG250.002102673536.015
GCCAATT250.002102673536.023
GCCGTCT1700.034.41176645
ATGCCGT92000.032.01358844
GCCCACG95050.031.03366918
CCGAGCC95150.031.02469614
CCCACGA94950.031.01895719
GAGCCCA95200.031.00840416
CGAGCCC95200.031.00840415
CACGAGA95000.031.00263221
TATGCCG95000.031.00263243
ACGAGAC95100.030.9936922
TCCGAGC95250.030.99212513
CCACGAG95050.030.98632420
AGCCCAC95150.030.97740217
CTCCGAG95250.030.96850212
TCGTATG95050.030.96265240
ATCTCGT94650.030.95087237
Produced by FastQC (version 0.11.2)