FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_3.351000000443ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_3.351000000443ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100277
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC2955429.472361558483No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT47124.698983814832912RNA PCR Primer, Index 44 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG9310.928428253737148No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT3890.3879254465131586RNA PCR Primer, Index 44 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC2120.2114143821614129RNA PCR Primer, Index 37 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCTA207.0024835E-445.018
TGCCGTC31250.043.84800345
ATGCCGT36150.037.78008744
TATGCCG36950.036.84032443
TCTCGTA36950.036.84032438
CGTATGC36850.036.81818441
TCGTATG36950.036.7794340
TATCTCG37200.036.77419336
GCCCACG37450.036.76902418
CCCACGA37450.036.76902419
CACGAGA37400.036.75802221
ATCTCGT37100.036.75201837
TCCGAGC37350.036.74698613
ACGAGAC37350.036.74698622
CTCGTAT37050.036.74089439
GACCCTA37300.036.73592426
GAGCCCA37550.036.73102616
GTATGCC37000.036.7297342
CCCTAGA37250.036.72483428
ACCCTAG37250.036.72483427
Produced by FastQC (version 0.11.2)