FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_29.351000000446d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_29.351000000446d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325890
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC7716323.677621283255085No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT113943.4962717481358743No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG42911.3167019546472736No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT10200.3129890453834116No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT8390.25744883242811994No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT7560.231980115990058No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC6890.21142103163644174No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT6410.19669213538310473No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA6110.18748657522476908No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA5910.181349535119212No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT5870.18012212709810058No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT5850.17950842308754486No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC5720.17551934701893276No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG5720.17551934701893276No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG5170.15864248672865078No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA5040.15465341066003868No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA4800.14728896253337015No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA4720.14483414649114731No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT4630.14207247844364662No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT4520.1386971063855902No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA4290.13163951026419957No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA4170.1279572862008653No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC4050.12427506213753108No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA4030.12366135812697536No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC4020.1233545061216975No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATCT3880.11905857804780755No Hit
AAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTT3840.11783117002669612No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC3740.11476264997391757No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT3680.11292153794225045No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA3650.11200098192641689No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATC3590.11015986989474977No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT3560.1092393138789162No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC3500.10739820184724908No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCT3470.1064776458314155No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC3440.10555708981558196No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC3370.10340912577863698No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA3340.1024885697628034No Hit
GAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTC3270.10034060572585843No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACA207.027492E-444.99785231
GTGGCCA207.027492E-444.99785213
ATCCGAC207.027492E-444.99785230
TCTCGAG207.027492E-444.99785211
TGCCGTC77800.044.27487645
ATGCCGT90150.038.18453644
TATGCCG94700.036.32614543
GTATGCC94800.036.31155842
TCGTATG94800.036.24035640
CGTATGC95000.036.2114341
CTCGTAT94900.036.20216839
GACATCT94650.036.131434
ATCTCGT94850.036.1263737
TCTCGTA94950.036.0646338
CATCTCG95100.036.0550636
CAGACAT94700.036.04104632
ATCAGAC94800.036.0267630
GTATAGA250.002099055636.020391
TAGATGT250.002102221836.009334
AGATGTT250.002102221836.009335
Produced by FastQC (version 0.11.2)