FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_25.3510000004465a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_25.3510000004465a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104119
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC1360613.067739797731443No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT20451.964098771597883No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG3640.34959997694945205No Hit
TTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCT3580.3438373399667688No Hit
ATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCTTC2340.22474284232464775Illumina PCR Primer Index 3 (95% over 22bp)
TTCTCTTTCCCTTTCTTTCTCTTTCTCTTTCCCTTTTTCTCTTTGTGTCTT2010.19304833891988976No Hit
GTTTCTTTTCGATTCGTTTTGATTCGATTCTTTTTGATTCATGTCGATTCG1210.1162131791507794No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAT207.0040976E-444.999528
TCGTGAA207.0040976E-444.999528
GCTACTT207.0040976E-444.9995244
TGGGCAA253.8665705E-544.9995233
TAGCTTA207.0040976E-444.9995215
ATGCACT253.8665705E-544.9995244
TTGGGCA207.0040976E-444.9995232
TGCCGTC14700.043.00974745
TACTGTG356.194172E-638.571027
GGGCAAA301.13287664E-437.499634
CAGGTAG250.002098446535.99961540
TAAAGGT250.002098446535.9996155
CGTGAAT250.002098446535.9996159
TTCGTGA250.002098446535.9996157
ATCACAG250.002098446535.99961536
AGCTGTG250.002098446535.99961531
ATTGGGC250.002098446535.99961531
TCTGGAG250.002098446535.99961540
GAAGGAC250.002098446535.99961522
ATGCCGT17600.035.92291344
Produced by FastQC (version 0.11.2)