H23LNBCXX_l01n01_bir_0113_pb_24.35100000044630.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0113_pb_24.35100000044630.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22360
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC	5414	24.2128801431127	RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT	473	2.1153846153846154	No Hit
TTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCT	211	0.943649373881932	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG	107	0.47853309481216455	No Hit
ATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCTTC	89	0.39803220035778175	RNA PCR Primer, Index 23 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCTT	49	0.21914132379248658	RNA PCR Primer, Index 23 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	600	0.0	40.875004	45
ATGCCGT	645	0.0	38.02326	44
AGCCCAC	660	0.0	37.5	17
GCCCACG	660	0.0	37.5	18
CACGAGA	660	0.0	37.5	21
ACGAGAG	660	0.0	37.5	27
AGACGAG	660	0.0	37.5	25
GACGAGA	660	0.0	37.5	26
GAGAGTT	660	0.0	37.5	29
CGAGAGT	660	0.0	37.5	28
CGAGACG	660	0.0	37.5	23
CCGTCTT	30	1.1045115E-4	37.499996	44
GATCTCG	650	0.0	37.384617	36
ATCTCGT	645	0.0	37.325584	37
TCTCGTA	645	0.0	37.325584	38
CCACGAG	665	0.0	37.21805	20
GAGCCCA	665	0.0	37.21805	16
CGAGCCC	665	0.0	37.21805	15
GTTGATC	660	0.0	37.159092	33
TGATCTC	660	0.0	37.159092	35