FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_23.35100000044616.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_23.35100000044616.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115458
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC2829324.505014810580473RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT91267.90417294600634No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG14881.2887803357064906No Hit
TTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCT5010.4339240243205322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC31050.043.4761745
GTCCTGG250.002099779435.9982743
CGTCTTC250.002099779435.9982745
AGTGATA250.002099779435.998278
TGATTAG250.002099779435.9982718
GATTAAC250.002099779435.9982716
ATGCCGT40250.033.53875744
GTTCTAT352.7984538E-432.155243
TATGCCG42000.032.1413143
CCCACGA42150.032.13368619
ACGAGAG42150.032.13368627
CCACGAG42150.032.13368620
GATCTCG41800.032.1336236
CGAGAGT42100.032.1184128
AGCCCAC42250.032.1108817
CACGAGA42250.032.1108821
AGACGAG42250.032.1108825
CCGAGCC42250.032.1108814
GAGCCCA42250.032.1108816
GCCCACG42200.032.0956118
Produced by FastQC (version 0.11.2)