FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_22.351000000445fb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_22.351000000445fb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11970
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC199216.641604010025063RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT4193.500417710944027No Hit
TTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCT680.5680868838763575No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG580.48454469507101083No Hit
ATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCTTC230.19214703425229743RNA PCR Primer, Index 23 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCTT170.14202172096908938RNA PCR Primer, Index 23 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG160.1336675020885547No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1950.042.69230745
AGCCCAC2250.037.017
GCCCACG2250.037.018
GAGCCCA2250.037.016
CCGAGCC2250.037.014
TCCGAGC2250.037.013
ATCTCCG2300.036.19565610
CCCACGA2300.036.19565619
AGACGAG2300.036.19565625
ATGCCGT2300.036.19565644
TTGATCT2300.036.19565634
CGAGCCC2300.036.19565615
GAGACGA2300.036.19565624
ATCTCGT2250.036.037
TCTCGTA2250.036.038
ACGAGAC2250.036.022
GATCTCG2250.036.036
TCTCCGA2250.036.011
CGAGACG2250.036.023
CTCGTAT2250.036.039
Produced by FastQC (version 0.11.2)