H23LNBCXX_l01n01_bir_0113_pb_21.351000000445d1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0113_pb_21.351000000445d1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23064
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC	382	1.6562608394033993	RNA PCR Primer, Index 23 (95% over 21bp)
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC	63	0.27315296566077	No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC	61	0.2644814429413805	No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG	51	0.22112382934443287	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG	40	0.1734304543877905	No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC	40	0.1734304543877905	No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA	36	0.15608740894901144	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT	34	0.14741588622962193	No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA	31	0.13440860215053765	No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC	31	0.13440860215053765	No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATACACATCTCCG	31	0.13440860215053765	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCT	30	0.1300728407908429	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	28	0.12140131807145335	No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATA	27	0.11706555671175858	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27	0.11706555671175858	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC	27	0.11706555671175858	No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT	27	0.11706555671175858	No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATA	26	0.11272979535206383	No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA	26	0.11272979535206383	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCT	25	0.10839403399236906	No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC	25	0.10839403399236906	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25	0.10839403399236906	No Hit
ATCCTACACAGATCGGTTCAAAGTCCAATGCAAAGCTACAGTAAAGGTTCA	24	0.10405827263267431	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24	0.10405827263267431	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	45	1.7915227E-8	40.0	45
AAAAGTA	40	1.49552525E-5	33.75	2
ATGCCGT	60	2.2498352E-7	29.999998	44
CCCACGA	70	2.6366251E-8	28.92857	19
AAAAAGT	40	5.983291E-4	28.125	1
CCACGAG	65	4.534304E-7	27.692307	20
GAGTTGA	65	4.534304E-7	27.692307	31
AGAGTTG	65	4.534304E-7	27.692307	30
ACGAGAG	65	4.534304E-7	27.692307	27
GATCTCG	65	4.534304E-7	27.692307	36
GAGAGTT	65	4.534304E-7	27.692307	29
CGAGAGT	65	4.534304E-7	27.692307	28
CGAGACG	65	4.534304E-7	27.692307	23
GAGACGA	65	4.534304E-7	27.692307	24
AAAGTAC	50	6.863898E-5	27.0	3
ATCTCGT	70	8.663119E-7	25.714285	37
TGATCTC	70	8.663119E-7	25.714285	35
TATGCCG	70	8.663119E-7	25.714285	43
CACGAGA	70	8.663119E-7	25.714285	21
AGACGAG	70	8.663119E-7	25.714285	25

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content