FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_19.3510000004459e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_19.3510000004459e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATTATTTGTTTTGAGGTTAGTTTGATTAGTCATTGTTCTGTCTCTTAT11.8181818181818181No Hit
GTATCAACGCAGAGTACGGGATGGTATCAACGCCTGTCTCTTATACACATC11.8181818181818181No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCTTA11.8181818181818181No Hit
GTCACATCATCAAACGAGATGTTCCCGTTATGCTTAATGTCTGTCTCTTAT11.8181818181818181No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT11.8181818181818181No Hit
ATCCAACATCAACATCTTGGTCAGATTTGAACTCTTCAATCTCTTGCACTC11.8181818181818181No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTT11.8181818181818181No Hit
GTAGAGAAATATCCAACTTAAATAGCGAAAAAGTGCACCATAATTACCTGT11.8181818181818181No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATCACGGAAC11.8181818181818181Illumina PCR Primer Index 8 (96% over 27bp)
CTCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG11.8181818181818181No Hit
GAGTACGGGGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA11.8181818181818181No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG11.8181818181818181No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACACGGCGTTAAACCC11.8181818181818181No Hit
AAGTCTCACTCTGTCACTCAAAAATAGAACAAAAAAGGCAAATATGACAAC11.8181818181818181No Hit
TCTCTAAACTTTATGCAGTACATCAAGAAGGAAATAAAAACGTTGGATTAG11.8181818181818181No Hit
TATCATTCTTGCCTTGAATGCGTATGATGAAAACCAGGTCAGATTTAACTG11.8181818181818181No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCAT11.8181818181818181No Hit
CTGTTAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGC11.8181818181818181No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG11.8181818181818181No Hit
GAACAGAAAGGATAAGGATGCTAAATTCCGTCTGATTCTAATAGAGAGCCG11.8181818181818181No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTTATCTCGTATGCCGTC11.8181818181818181No Hit
GCTTTGTGATGGATCATTAAGAATATCCTCAAATCCAATAGTCTCATCATT11.8181818181818181No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT11.8181818181818181No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAGTACCTGTC11.8181818181818181No Hit
AAAATGCACATATTGTGAGCTCGATGAGTAGCTGTCTCTTATACACATCTC11.8181818181818181No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCT11.8181818181818181No Hit
ATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA11.8181818181818181No Hit
GCATGGACGAAATCAGAAATGCAGGAGTGATTCATTACAATGGAAACATGA11.8181818181818181No Hit
AAAAAGTACTCTGCGTTGATACCATCCCGTACTCTGCGTTGATACCTGTCT11.8181818181818181No Hit
GAAGAGTCAATGAACTCGAATGGAATCATCATTGAATGGAATCGAATGGAA11.8181818181818181No Hit
CAGGTACTTTTTTTTTTTTTTTTTTTGCGTTGATACCACCCGTACCCGTCC11.8181818181818181No Hit
GATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGC11.8181818181818181No Hit
TATCAAAAGAAGTCAGGACAGAGTATGTACTAGGGGGTTCTATTTATATGC11.8181818181818181No Hit
TTGTTCGTGAATGTAAATGCAAAAATTACCTACCTGTCTCTTATACACATC11.8181818181818181No Hit
GGTATCAACGCAGAGTACGGGGCAGAGTACTTTTTTTAAAAAAAAAAACAA11.8181818181818181No Hit
TTTCAGTATAGTAGCAGCATCATTTGTTACAAAGAGTTTTTCAATGTGGTT11.8181818181818181No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC11.8181818181818181RNA PCR Primer, Index 23 (95% over 21bp)
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAACTGTCTCTTAT11.8181818181818181No Hit
GAGTTACTATGGAGTTAGCTTTTAAAAAATTTTCTGCGTCTCTAAACCTGT11.8181818181818181No Hit
CATATGGATTTTCCATCAAAAGTTTCTTTAGCTTCTTTAACTTGGGTCTAC11.8181818181818181No Hit
AGATGAAAGACACTGATTCAGAGGAAGAGCTTAAGGACTGTCTCTTATACA11.8181818181818181No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT11.8181818181818181No Hit
GAATAGTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCTGTCTC11.8181818181818181No Hit
ACTTTCCACTCTTCATCACAAGAGCTAATCTGCTGTTGATTCCCTGTCTCT11.8181818181818181No Hit
CATTAGAGATGAATTTTGGGATCCTATCACTCACAACTCCAAAATCCATAA11.8181818181818181No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGAGTTGATCTCGTATGCCGT11.8181818181818181No Hit
AAAAAGTACTCTGCGTCCCGTACTCTGCGTTGATCTGTCTCTTATACACAT11.8181818181818181No Hit
CACATTGACTGTCTGTAGATTTTTGAAAGTGGTAACAGGTACATAGGTAAC11.8181818181818181No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC11.8181818181818181No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAACAAATA11.8181818181818181No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCACCAGAAAAAACCTAAA11.8181818181818181No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.8181818181818181No Hit
CCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACCTGTCTCTTAT11.8181818181818181No Hit
GGACAGCTCAGCATCAGGGGTAGCTTCCACGCGAGCAGTACCGCCTGGTTC11.8181818181818181No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACACAACTC11.8181818181818181RNA PCR Primer, Index 36 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAA50.045.018
TGGTATC50.045.011
CGGGGTG50.045.06
ACGCAAA50.045.019
TACGGGG50.045.04
GTATCAA50.045.013
TCAACGC50.045.016
AGTACGG50.045.02
GCAAAAA50.045.021
GAGTACG50.045.01
ACGGGGT50.045.05
TATCAAC50.045.014
GGTGGTA50.045.09
GGGGTGG50.045.07
GTGGTAT50.045.010
ATCAACG50.045.015
GGGTGGT50.045.08
GGTATCA50.045.012
CGCAAAA50.045.020
GTACGGG50.045.03