H23LNBCXX_l01n01_bir_0113_pb_16.35100000044531.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0113_pb_16.35100000044531.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24448
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC	7242	29.62205497382199	RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT	699	2.8591295811518322	No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT	207	0.8466950261780105	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG	201	0.8221531413612565	No Hit
ATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTTC	45	0.18406413612565445	RNA PCR Primer, Index 45 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTT	30	0.12270942408376963	RNA PCR Primer, Index 45 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	775	43.258064	45
ATGCCGT	880	38.096592	44
TATGCCG	905	37.044197	43
CGTATGC	905	37.044197	41
AATCTCG	900	37.000004	36
ATCTCGT	900	37.000004	37
TCTCGTA	900	37.000004	38
TAATCTC	900	37.000004	35
TCGTATG	900	37.000004	40
CCACGAG	915	36.885242	20
CCCACGA	915	36.885242	19
ACGAGAC	915	36.885242	22
GTATGCC	910	36.84066	42
GACGTTC	910	36.84066	26
TTAATCT	910	36.84066	34
CGTTCAT	905	36.795578	28
ACGTTCA	905	36.795578	27
GTTCATT	905	36.795578	29
CTCGTAT	905	36.795578	39
AGCCCAC	920	36.68478	17