FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_15.35100000044517.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_15.35100000044517.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39896
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC1413535.429617004210954RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT431010.803088028875075No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG11772.950170443152196No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT1150.28824944856627227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC15400.043.53774645
GCCGTCT501.02591E-940.4988745
ATGCCGT19700.033.8061644
TTAATCT21700.030.79407134
AATCTCG21700.030.69038836
TATGCCG21700.030.69038843
TAATCTC21700.030.69038835
ATTAATC21800.030.65281533
TCCGAGC21950.030.64834813
GCCCACG21750.030.61983318
CTCGTAT21700.030.58670439
GAGCCCA21850.030.58267216
AGCCCAC21800.030.54960617
ATCTCGT21800.030.54960637
ACGAGAC21950.030.54584522
CTCCGAG21950.030.54584512
CATTAAT21900.030.51284832
CGAGCCC21900.030.51284815
GAGACGT21900.030.51284824
TCTCCGA22050.030.50935411
Produced by FastQC (version 0.11.2)